Product Information

Contents: Affinity Purified anti-human CD11c
Catalog Number: 14-0116
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 3.9
Isotype: Mouse IgG1, κ
HLDA No.: III NL707

Surface staining of normal human peripheral blood cells with anti-human CD11c (3.9) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0116	FITC anti-human CD11c	488	518	FC
12-0116	PE anti-human CD11c	488	575	FC
17-0116	Allophycocyanin (APC) anti-human CD11c	633	660	FC
25-0116	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD11c	488	760	FC
56-0116	Alexa Fluor® 700 anti-human CD11c (APC-Cy5.5 Equivalent)	633	723	FC
14-0116	Affinity Purified anti-human CD11c	N/A	N/A	FC IH/F
13-0116	Biotin anti-human CD11c	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin α_X chain. CD11c non-covalently associates with β₂ integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 3.9 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The 3.9 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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