Product Information

Contents: Affinity Purified anti-human mature macrophage marker (late-stage)
Catalog Number: 14-0115
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio25F9 (25F9)
Isotype: Mouse IgG1

Staining of cultured macrophages from peripheral blood monocytes with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human mature macrophage marker (25F9) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SaV-APC (cat. 17-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0115	Affinity Purified anti-human mature macrophage marker (late-stage)	N/A	N/A	FC IH/F IHC(Paraffin) IP WB
13-0115	Biotin anti-human mature macrophage marker (late-stage)	N/A	N/A	FC
51-0115	Alexa Fluor® 647 anti-human mature macrophage marker (late-stage)	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody 25F9 recognizes a protein on mature macrophages both on the cell surface and in intracellular vesicular structures. Expression is thought to correlate with endocytic macrophages and is useful in many disease models. Expression is not found on immature macrophages or monocytes or any other cell hematopoietic cell.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio25F9 (25F9) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen and paraffin embedded tissue sections.

Applications Tested

This eBio25F9 (25F9) antibody has been tested by flow cytometric analysis of human macrophages. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control