Anti-Mouse CD11c Purified

Also known as: Integrin alpha X, Integrin aX, ITGAX, p150/95, leu M5 alpha

RUO: For Research Use Only

CD11c : mouse CD11c antibody purified eBioscience

Staining of mouse splenocytes with Anti-Mouse CD11c FITC (left) and PE (right), and Anti-Human/Mouse CD45R (B220) APC. Total viable cells were used for analysis.

Contents: Anti-Mouse CD11c Purified
Catalog Number: 14-0114
Concentration: 0.5 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 2-8°C.
Clone: N418
Host/Isotype: Armenian Hamster IgG

Product Options
Cat. No.	Size	Price	Add Qty to Cart
14-0114-81	50 ug	To view relevant pricing, please select your country and resume browsing.
14-0114-82	100 ug
14-0114-85	500 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4111	Anti-Armenian Hamster IgG FITC
11-4317	Streptavidin FITC
12-4317	Streptavidin PE
13-4113	Anti-Armenian Hamster IgG Biotin (Polyclonal)
14-4888	Armenian Hamster IgG Isotype Control Purified (eBio299Arm)
17-4317	Streptavidin APC

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-0114	Anti-Mouse CD11c FITC	488	518	FC	RUO
12-0114	Anti-Mouse CD11c PE	488	575	FC	RUO
13-0114	Anti-Mouse CD11c Biotin	N/A	N/A	FC	RUO
14-0114	Anti-Mouse CD11c Purified	N/A	N/A	FC IHC (F) IP	RUO
15-0114	Anti-Mouse CD11c PE-Cy5	488	670	FC	RUO
16-0114	Anti-Mouse CD11c Functional Grade* Purified	N/A	N/A	FC	RUO
17-0114	Anti-Mouse CD11c APC	633	660	FC	RUO
25-0114	Anti-Mouse CD11c PE-Cy7	488	760	FC	RUO
35-0114	Anti-Mouse CD11c PE-Cy5.5 (To Be Discontinued; See Replacement Formats: PerCP-eFluor® 710 or PerCP-Cy5.5)	488	690	FC	RUO
45-0114	Anti-Mouse CD11c PerCP-Cy5.5 (PE-Cy5.5 replacement)	488	690	FC	RUO
47-0114	Anti-Mouse CD11c APC-eFluor® 780	633	780	FC	RUO
48-0114	Anti-Mouse CD11c eFluor® 450 (Pacific Blue® replacement)	405	450	FC	RUO
51-0114	Anti-Mouse CD11c Alexa Fluor® 647	633	668	FC	RUO
53-0114	Anti-Mouse CD11c Alexa Fluor® 488	488	519	FC	RUO
56-0114	Anti-Mouse CD11c Alexa Fluor® 700	633	723	FC	RUO
94-0114	Anti-Mouse CD11c eFluor® 625NC	350-500	625	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The N418 monoclonal antibody reacts with mouse CD11c, the integrin α_X. CD11c non-covalently associates with β₂ integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The N418 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The N418 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Xin KQ, Mizukami H, Urabe M, Toda Y, Shinoda K, Yoshida A, Oomura K, Kojima Y, Ichino M, Klinman D, Ozawa K, Okuda K. Induction of robust immune responses against human immunodeficiency virus is supported by the inherent tropism of adeno-associated virus type 5 for dendritic cells. J Virol. 2006 Dec;80(24):11899-910. (N418, FC, PubMed)

Ohteki T, Tada H, Ishida K, Sato T, Maki C, Yamada T, Hamuro J, Koyasu S. Essential roles of DC-derived IL-15 as a mediator of inflammatory responses in vivo. J Exp Med. 2006 Oct 2;203(10):2329-38. (N418, IHC frozen, PubMed)

Zhang J, Kawashima N, Suda H, Nakano Y, Takano Y, Azuma M. The existence of CD11c+ sentinel and F4/80+ interstitial dendritic cells in dental pulp and their dynamics and functional properties. Int Immunol. 2006 Sep;18(9):1375-84. (N418, IHC frozen, PubMed)

Guiducci C, Vicari AP, Sangaletti S, Trinchieri G, Colombo MP. Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection. Cancer Res. 2005 Apr 15;65(8):3437-46. (N418, IHC frozen, PubMed)

Esche C, Gambotto A, Satoh Y, Gerein V, Robbins PD, Watkins SC, Lotze MT, Shurin MR. CD154 inhibits tumor-induced apoptosis in dendritic cells and tumor growth.Eur J Immunol. 1999 Jul;29(7):2148-55.

Finkelman FD, Lees A, Birnbaum R, Gause WC, Morris SC. Dendritic cells can present antigen in vivo in a tolerogenic or immunogenic fashion.J Immunol. 1996 Aug 15;157(4):1406-14.

Crowley MT, Inaba K, Witmer-Pack MD, Gezelter S, Steinman RM. Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen. J Immunol Methods. 1990 Oct 4;133(1):55-66.

Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, Steinman RM. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J Exp Med. 1990 May 1;171(5):1753-71.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.