Product Information

Contents: Affinity Purified anti-human CD11b (Integrin α_M, Mac-1) - activation epitope
Catalog Number: 14-0113
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: CBRM1/5
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0113	FITC anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	488	518	FC
12-0113	PE anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	488	575	FC
14-0113	Affinity Purified anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	N/A	N/A	FA FC IP
16-0113	Functional Grade* Purified anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	N/A	N/A	FA FC
51-0113	Alexa Fluor® 647 anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	633	668	FC
56-0113	Alexa Fluor® 700 anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope (APC-Cy5.5 Equivalent)	633	723	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the α chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CBRM1/5 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. CBRM1/5 has also been reported in blocking of CD11b function. (Please use Functional Grade purified CBRM1/5, cat. 16-0113, in functional assays.)

Applications Tested

The CBRM1/5 antibody has been tested by flow cytometric analysis of resting and activated human peripheral leukocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Oxvig, C., C. Lu, et al. 1999. Conformational changes in tertiary structure near the ligand binding site of an integrin I domain. Proc Natl Acad Sci U S A 96(5): 2215-20.
Weber, C., J. Kitayama, et al. 1996. Differential regulation of beta 1 and beta 2 integrin avidity by chemoattractants in eosinophils. Proc Natl Acad Sci U S A 93(20): 10939-44.
Diamond, M. S. and T. A. Springer 1993. A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen. J Cell Biol 120(2): 545-56.

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