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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin αM. CD11b non-covalently associates with CD18 to form αMβ2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions. Applications ReportedFor research use only, not for diagnostic or therapeutic use. The M1/70 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. M1/70 has also been reported in in vitro blocking of CD11b function. (Please use Functional Grade purified M1/70, cat. 16-0112, in functional assays.) Applications TestedThe M1/70 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. References
\Springer, T., G. Galfre, et al. 1978. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol 8(8): 539-51. Springer, T., G. Galfre, et al. 1979. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol 9(4): 301-6. Sanchez-Madrid, F., P. Simon, et al. 1983. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med 158(2): 586-602. Ault KA and Springer TA. 1981. Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 126(1):359-64. (cross-reactivity to human, PubMed) Dembic Z, Schenck K, and Bogen B. 2000. Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells. Proc Natl Acad Sci U S A. 97(6):2697-702. (IHC frozen, PubMed) Zhang Y, McCormick LL, et al. 2002. Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol. 168(6):3088-98. (IHC frozen, PubMed) Whiteland JL, Nicholls SM, et al. 1995. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem. 43(3):313-20. (IHC paraffin, PubMed) Related ProductsCat. 11-0117 NEW CATALOG#, SEE 11-0112, FITC anti-m/h CD11b (integrin alpha M, Mac-1 alpha) (clone M1/70) Cat. 14-4031 Purified Rat IgG2b Isotype Control Cat. 11-4317 Streptavidin-FITC (Fluorescein isothiocyanate) Cat. 12-4317 Streptavidin-PE (Phycoerythrin) Cat. 17-4317 Streptavidin Allophycocyanin (SA-APC) Cat. 11-4811 FITC Anti-Rat IgG Cat. 13-4813 Biotin Anti-Rat IgG (clone Polyclonal) |
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Copyright © 2000-2008 eBioscience, Inc. Product For Research Use Only: Not for further distribution without written consent. |
Mouse Cellular Antigens Chart
