Surface staining of mouse splenocytes with anti-mouse CD11a (M17/4) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Contents: Affinity Purified anti-mouse CD11a (Integrin αL, LFA-1α) Catalog Number: 14-0111 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Clone: M17/4 Host/Isotype: Rat IgG2a, κ Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles.
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Cat. No.
Size
Price
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14-0111-81
50 ug
14-0111-82
100 ug
14-0111-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin αL, also known as the lymphocyte function associated antigen-1 (LFA-1) α chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin β2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The M17/4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. M17/4 has also been reported in blocking of CD11a function. (Please use Functional Grade purified M17/4, cat. 16-0111, in functional assays.)
Applications Tested
The M17/4 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Sanchez-Madrid, F., P. Simon, et al. 1983. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med 158(2): 586-602. Sanchez-Madrid, F., D. Davignon, et al. 1982. Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell Immunol 73(1): 1-11.
