Product Information

Contents: Affinity Purified anti-human CD10 (CALLA)
Catalog Number: 14-0108
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioSN5c (SN5c L4-1A1)
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CD10 (SN5c) (colored histogram) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (cat. 12-4012). Cells in the granulocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0108	Affinity Purified anti-human CD10 (CALLA)	N/A	N/A	FC IH/F IP WB
13-0108	Biotin anti-human CD10 (CALLA)	N/A	N/A	FC IH/F

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioSN5c monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALL). Subsequently, CD10 was shown to be the same molecule as the neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn2+-dependent metallo-peptidase with endothelin, glucagon, gastrin, neurotensin and bradykinin included among its substrates. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and expression has also been reported on mature B cells in germinal centers. Expression is found on normal donor granulocytes and bone marrow stromal cells. Most likely CB-CALLA and eBioSN5c see different epitopes due to there ability to co-stain.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioSN5c (SN5c L4-1A1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections.

Applications Tested

This eBioSN5c (SN5c L4-1A1) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Biddle WC, Haruta Y, Seon BK, Henderson ES, Sarcione EJ. In vitro and in vivo cytotoxic activity of anti-human leukemia monoclonal antibodies SN5c and SN6 daunorubicin conjugates. Leuk Res. 1989;13(8):699-707.

Matsuzaki H, Haruta Y, Fukukawa T, Barcos MP, Seon BK. Unique epitopes of common acute lymphoblastic leukemia antigen detected by new monoclonal antibodies. Cancer Res. 1987 Apr 15;47(8):2160-6 PubMed.

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