Also known as: Common Acute Lymphocytic Leukemia antigen (CALLA), Neprilysin,
RUO: For Research Use Only
Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 K Iso Cntrl Purified (cat. 14-4714) (open histogram) or 0.25 µg of Anti-Human CD10 Purified (filled histogram) followed by F(ab') Anti-Mouse IgG PE (cat. 12-4012). Cells in the granulocyte gate were used for analysis.
Contents: Anti-Human CD10 Purified Catalog Number: 14-0108 Concentration: 0.5 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Clone: SN5c Host/Isotype: Mouse IgG1
Product Options
Cat. No.
Size
Price
Add Qty to Cart
14-0108-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBioSN5c monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALLs). Subsequently, CD10 was shown to be the same molecule as neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn2+-dependent metallopeptidase acting on substrates such as endothelin, glucagon, gastrin, neurotensin and bradykinin. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and its expression has been reported on mature germinal center B cells. CD10 expression is also found on normal donor granulocytes and bone marrow stromal cells. Most likely, CB-CALLA and eBioSN5c see different epitopes due to their ability to co-stain.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioSN5c (SN5c L4-1A1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections.
Applications Tested
This eBioSN5c (SN5c L4-1A1) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Biddle WC, Haruta Y, Seon BK, Henderson ES, Sarcione EJ. In vitro and in vivo cytotoxic activity of anti-human leukemia monoclonal antibodies SN5c and SN6 daunorubicin conjugates. Leuk Res. 1989;13(8):699-707.
Matsuzaki H, Haruta Y, Fukukawa T, Barcos MP, Seon BK. Unique epitopes of common acute lymphoblastic leukemia antigen detected by new monoclonal antibodies. Cancer Res. 1987 Apr 15;47(8):2160-6. (PubMed)
