Product Information

Contents: Affinity Purified anti-human CD10 (CALLA, NEP, enkephalinase, Neprilysin)
Catalog Number: 14-0106
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioCB-CALLA (CB-CALLA)
Isotype: Mouse IgG2b

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (open histogram) or 0.125 μg of Purified anti-human CD10 (CB-CALLA) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and Streptavidin-PE (cat. 12-4317). Cells in the granulocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0106	Affinity Purified anti-human CD10 (CALLA, NEP, enkephalinase, Neprilysin)	N/A	N/A	FC
13-0106	Biotin anti-human CD10 (CALLA, NEP, enkephalinase, Neprilysin)	N/A	N/A	FC
11-0106	Fluorescein isothiocyanate (FITC) anti-human CD10 (CALLA, NEP, enkephalinase, Neprilysin)	488	518	FC
12-0106	Phycoerythrin (PE) anti-human CD10 (CALLA, NEP, enkephalinase, Neprilysin)	488	575	FC
15-0106	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD10 (CALLA, NEP, enkephalinase, Neprilysin)	488	670	FC
17-0106	Allophycocyanin (APC) anti-human CD10 (CALLA, NEP, enkephalinase, Neprilysin)	633	660	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioCB-CALLA monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALL). Subsequently, CD10 was shown to be the same molecule as the neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn2+-dependent metallo-peptidase with endothelin, glucagon, gastrin, neurotensin and bradykinin included among its substrates. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and expression has also been reported on mature B cells in germinal centres. In addition to the hematopoietic compartment, other major sites of CD10 expression are the brush border of enterocytes and renal tubules and glomeruli. There is partial blocking of the eBioCB-CALLA and MEM-78 monoclonal antibodies indicating that they recognize similar epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioCB-CALLA (CB-CALLA) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioCB-CALLA (CB-CALLA) antibody has been tested by flow cytometric analysis of normal human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ritz J, Pesando JM, Notis-McConarty J, Lazarus H, Schlossman SF. A monoclonal antibody to human acute lymphoblastic leukaemia antigen. Nature. 1980 Feb 7;283(5747):583-5.

McCormack RT, Nelson RD, LeBien TW. Structure/function studies of the common acute lymphoblastic leukemia antigen (CALLA/CD10) expressed on human neutrophils. J Immunol. 1986 Aug 1;137(3):1075-82.

Pesando JM, Tomaselli KJ, Lazarus H, Schlossman SF. Distribution and modulation of a human leukemia-associated antigen (CALLA). J Immunol. 1983 Oct;131(4):2038-45.

Related Products

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4732    Affinity Purified Mouse IgG2b Isotype Control