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Product Information

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Contents: Affinity Purified anti-human CD8a Catalog Number: 14-0088 Sizes: 25 ug, 100 ug Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: RPA-T8 Isotype: Mouse IgG1, κ HLDA No.: IV T171

Profile of purified mAb RPA-T8 (CD8) staining on human peripheral blood lymphocytes and detection with FITC anti-mouse IgG (cat.no. 11-4011)

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0088	Affinity Purified anti-human CD8 (CD8a; alpha chain)	N/A	N/A	FC  IH/F
11-0088	FITC anti-human CD8 (CD8a; alpha chain)	488	518	FC
12-0088	PE anti-human CD8 (CD8a; alpha chain)	488	575	FC
15-0088	PE-Cy5 anti-human CD8 (CD8a; alpha chain)	488	670	FC
17-0088	APC anti-human CD8 (CD8a; alpha chain)	633	660	FC
25-0088	PE-Cy7 anti-human CD8 (CD8a; alpha chain)	488	760	FC
45-0088	PerCP-Cy5.5 anti-human CD8 (CD8a; alpha chain) (PE-Cy5.5 replacement)	488	690	FC
93-0088	eFluorTM 605NC anti-human CD8 (CD8a; alpha chain)	350-500	605	FC
94-0088	eFluorTM 625NC anti-human CD8 (CD8a; alpha chain)	350-500	625	FC
95-0088	eFluorTM 650NC anti-human CD8 (CD8a; alpha chain)	350-500	650	FC
47-0088	Allophycocyanin-eFluorTM 780 anti-human CD8 (CD8a; alpha chain) (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0088	eFluorTM 450 anti-human CD8 (CD8a; alpha chain) (Pacific Blue® replacement)	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The RPA-T8 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

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Applications Tested

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The RPA-T8 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London.

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Related Products

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Cat. 11-0089  FITC anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 12-0089  PE anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 13-0089  Biotin anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 14-0089  Affinity Purified anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 16-0089  Functional Grade Purified anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
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Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
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