Product Information

Contents: Affinity Purified anti-human CD8a
Catalog Number: 14-0088
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: RPA-T8
Isotype: Mouse IgG1, κ
HLDA No.: IV T171

Profile of purified mAb RPA-T8 (CD8) staining on human peripheral blood lymphocytes and detection with FITC anti-mouse IgG (cat.no. 11-4011)

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0088	FITC anti-human CD8 (CD8a; alpha chain)	488	518	FC
12-0088	PE anti-human CD8 (CD8a; alpha chain)	488	575	FC
15-0088	PE-Cy5 anti-human CD8 (CD8a; alpha chain)	488	670	FC
17-0088	APC anti-human CD8 (CD8a; alpha chain)	633	660	FC
25-0088	PE-Cy7 anti-human CD8 (CD8a; alpha chain)	488	760	FC
27-0088	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD8 (CD8a; alpha chain) (APC-Cy7 Equivalent)	633	779	FC
45-0088	PerCP-Cy5.5 anti-human CD8 (CD8a; alpha chain)	488	690	FC
94-0088	eFluor^TM 625^NC anti-human CD8 (CD8a; alpha chain)	350-500	625	FC
14-0088	Affinity Purified anti-human CD8 (CD8a; alpha chain)	N/A	N/A	FC IH/F

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Description

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RPA-T8 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The RPA-T8 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London.

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