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Affinity Purified anti-human CD8a
 
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Adobe PDF    Human Cellular Antigens Chart
Contents: Affinity Purified anti-human CD8a
Catalog Number: 14-0088
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: RPA-T8
Isotype: Mouse IgG1, κ
HLDA No.: IV T171

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14-0088-80 25 ug
14-0088-82 100 ug


   
data image 1
Profile of purified mAb RPA-T8 (CD8) staining on human peripheral blood lymphocytes and detection with FITC anti-mouse IgG (cat.no. 11-4011)
Available Formats of This Product
Cat. No. Format Excite
(nm)		 Emit
(nm)		 Reported Applications
14-0088 Affinity Purified anti-human CD8 (CD8a; alpha chain) N/A N/A FC  IH/F 
11-0088 FITC anti-human CD8 (CD8a; alpha chain) 488 518 FC 
12-0088 PE anti-human CD8 (CD8a; alpha chain) 488 575 FC 
15-0088 PE-Cy5 anti-human CD8 (CD8a; alpha chain) 488 670 FC 
17-0088 APC anti-human CD8 (CD8a; alpha chain) 633 660 FC 
25-0088 PE-Cy7 anti-human CD8 (CD8a; alpha chain) 488 760 FC 
45-0088 PerCP-Cy5.5 anti-human CD8 (CD8a; alpha chain) (PE-Cy5.5 replacement) 488 690 FC 
93-0088 eFluorTM 605NC anti-human CD8 (CD8a; alpha chain) 350-500 605 FC 
94-0088 eFluorTM 625NC anti-human CD8 (CD8a; alpha chain) 350-500 625 FC 
95-0088 eFluorTM 650NC anti-human CD8 (CD8a; alpha chain) 350-500 650 FC 
47-0088 Allophycocyanin-eFluorTM 780 anti-human CD8 (CD8a; alpha chain) (APC-Alexa Fluor® 750 replacement) 633 780 FC 
48-0088 eFluorTM 450 anti-human CD8 (CD8a; alpha chain) (Pacific Blue® replacement) 405 450 FC 
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RPA-T8 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested

The RPA-T8 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References


Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London.

Related Products

Cat. 11-0089  FITC anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 12-0089  PE anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 13-0089  Biotin anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 14-0089  Affinity Purified anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 16-0089  Functional Grade Purified anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control
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