Anti-Mouse CD8a Purified

Also known as: CD8 alpha, Ly-2, Ly-35, Ly-B, Lyt-2

RUO: For Research Use Only

CD8 : mouse CD8 antibody Purified eBioscience

Staining of C57Bl/6 splenocytes with 0.125 µg of Rat IgG2a Isotype Control Purified (cat.14-4321) (open histogram) or 0.125 µg of Anti-Mouse CD8 Purified (filled histogram) followed by Anti-Rat IgG FITC (cat.11-4811). Total viable cells were used for analysis.

Contents: Anti-Mouse CD8a Purified
Catalog Number: 14-0081
Concentration: 0.5 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 2-8°C.
Clone: 53-6.7
Host/Isotype: Rat IgG2a, κ

Product Options
Cat. No.	Size	Price	Add Qty to Cart
14-0081-82	100 ug	To view relevant pricing, please select your country and resume browsing.
14-0081-85	500 ug
14-0081-86	1 mg

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4317	Streptavidin FITC
11-4811	Anti-Rat IgG FITC
12-4317	Streptavidin PE
13-4813	Anti-Rat IgG Biotin (Polyclonal)
14-4321	Rat IgG2a K Isotype Control Purified
17-4317	Streptavidin APC

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-0081	Anti-Mouse CD8a FITC	488	518	FC	RUO
12-0081	Anti-Mouse CD8a PE	488	575	FC	RUO
13-0081	Anti-Mouse CD8a Biotin	N/A	N/A	FC	RUO
14-0081	Anti-Mouse CD8a Purified	N/A	N/A	FC IHC (F) IP	RUO
15-0081	Anti-Mouse CD8a PE-Cy5	488	670	FC	RUO
16-0081	Anti-Mouse CD8a Functional Grade* Purified	N/A	N/A	FA FC	RUO
17-0081	Anti-Mouse CD8a APC	633	660	FC	RUO
19-0081	Anti-Mouse CD8a Cy5	633	670	FC	RUO
25-0081	Anti-Mouse CD8a PE-Cy7	488	760	FC	RUO
36-0081	Anti-Mouse CD8a Functional Grade* Biotin	N/A	N/A	FC IHC (F)	RUO
45-0081	Anti-Mouse CD8a PerCP-Cy5.5	488	690	FC	RUO
46-0081	Anti-Mouse CD8a PerCP-eFluor® 710	488	710	FC	RUO
47-0081	Anti-Mouse CD8a APC-eFluor® 780	633	780	FC	RUO
48-0081	Anti-Mouse CD8a eFluor® 450 (Pacific Blue® replacement)	405	450	FC	RUO
50-0081	Anti-Mouse CD8a eFluor® 660 (Alexa Fluor® 647 Replacement)	633	660	FC IHC (F)	RUO
51-0081	Anti-Mouse CD8a Alexa Fluor® 647 (To Be Discontinued. Refer to New Format: eFluor® 660 cat. 50-0081)	633	668	FC	RUO
53-0081	Anti-Mouse CD8a Alexa Fluor® 488	488	519	FC	RUO
56-0081	Anti-Mouse CD8a Alexa Fluor® 700	633	723	FC	RUO
93-0081	Anti-Mouse CD8a eFluor® 605NC	350-500	605	FC	RUO
95-0081	Anti-Mouse CD8a eFluor® 650NC	350-500	650	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature αβ TCR T cells express CD8 αβ while γδ TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 αα. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 53-6.7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. It has also been reported in T cell activation, isolation and depletion of CD8⁺ cells, and blocking of ligand binding. (Please use Functional Grade purified 53-6.7, cat. 16-0081, in functional assays.)

Applications Tested

The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S. Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. (53-6.7, in vivo depletion, PubMed)

Yang Z, Day YJ, Toufektsian MC, Xu Y, Ramos SI, Marshall MA, French BA, Linden J. Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. Circulation. 2006 Nov 7;114(19):2056-64. (53-6.7, in vivo depletion, PubMed)

Taylor JL, Ordway DJ, Troudt J, Gonzalez-Juarrero M, Basaraba RJ, Orme IM. Factors associated with severe granulomatous pneumonia in Mycobacterium tuberculosis-infected mice vaccinated therapeutically with hsp65 DNA. Infect Immun. 2005 Aug;73(8):5189-93. (53-6.7, IHC frozen)

Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K. Beta2 integrins are required for skin homing of primed T cells but not for priming naïve T cells. J Clin Invest. 2002 Jan;109(2):183-92. (53-6.7, IHC frozen)

Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280-95.

Ledbetter, J. A. and L. A. Herzenberg. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90.

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