Product Information

Contents: Affinity Purified anti-rat CD8b (beta subunit; CD8b, Ly-3)
Catalog Number: 14-0080
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio341 (341)
Isotype: Mouse IgG1, κ

Staining of Lou rat splenocytes with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-rat CD8b (341) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0080	Affinity Purified anti-rat CD8b (beta subunit; CD8b, Ly-3)	N/A	N/A	FA FC IHC IP WB
16-0080	Functional Grade* Purified anti-rat CD8b (beta subunit; CD8b, Ly-3)	N/A	N/A	FA FC IHC(Paraffin)
11-0080	Fluorescein isothiocyanate (FITC) anti-rat CD8b (beta subunit; CD8b, Ly-3)	488	518	FC
12-0080	Phycoerythrin (PE) anti-rat CD8b (beta subunit; CD8b, Ly-3)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio341 monoclonal antibody reacts with rat CD8β. CD8β is a member of the immunoglobulin superfamily which heterodimerizes with CD8α to form the CD8 antigen expressed on CD4+CD8+, double-positive thymocytes, the majority of MHC class I-restricted peripheral T cells, and on some macrophages. CD8β expression is absent in athymic mice, which are populated by T-cells expressing CD8α homodimers. This has led to the idea that CD8β expression is dependent on the development of T cells in the thymus. CD8 binds to MHC class I expressed on the surface of antigen-presenting cells during antigen presentation, and participates in T-cell receptor signal transduction through association with the kinase Lck.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio341 (341) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining. (Please use Functional Grade purified eBio341 (341), cat. 16-0080, in functional assays.)

Applications Tested

This eBio341 (341) antibody has been tested by flow cytometric analysis of rat splenocytes and thymocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hunig T. Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov;22(11):2841-8. (341, FC, FA, PubMed)

Kuhnlein P, Park JH, Herrmann T, Elbe A, Hunig T. Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J Immunol. 1994 Aug 1;153(3):979-86. (341, FC, PubMed)

Hirji N, Lin TJ, Bissonnette E, Belosevic M, Befus AD. Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J Immunol. 1998 Jun 15;160(12):6004-11. (341, FA, PubMed)

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