human CD5 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-human CD5
Catalog Number: 14-0059
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: UCHT2
Isotype: Mouse IgG1, κ
HLDA No.: III 518

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14-0059-80

25 ug

14-0059-82

100 ug

Surface staining of normal human peripheral blood cells with anti-human CD5 (UCHT2) FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0059	Affinity Purified anti-human CD5	N/A	N/A	FC IHC WB
13-0059	Biotin anti-human CD5	N/A	N/A	FC
11-0059	FITC anti-human CD5	488	518	FC
12-0059	PE anti-human CD5	488	575	FC
25-0059	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD5	488	760	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The UCHT2 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining.

Applications Tested

The UCHT2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

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