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Contents: Affinity Purified anti-rat CD5 Catalog Number: 14-0050 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: HIS47 Isotype: Mouse IgG2a, κ Prices for This Product* Cat. No. Size Price Qty Action 14-0050-82 100 ug $145 *International customers: Please contact your distributor for region specific pricing.

Surface staining of rat splenocytes with 0.06 μg anti-rat CD5 (HIS47) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0050	FITC anti-rat CD5	488	518	FC
12-0050	PE anti-rat CD5	488	575	FC
14-0050	Affinity Purified anti-rat CD5	N/A	N/A	FC  IH/F
15-0050	Phycoerythrin-Cy5 (PE-Cy5) anti-rat CD5	488	670	FC
17-0050	Allophycocyanin (APC) anti-rat CD5	633	660	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The HIS47 monoclonal antibody reacts with rat CD5, a 67 kDa surface protein expressed by a majority of thymocytes and mature T cells. While mouse and human CD5 is also expressed on a small subset of B cells called B-1 cells, such CD5⁺ B cell subset has not been identified in the rat so far. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The HIS47 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

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Applications Tested

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The HIS47 antibody has been tested by flow cytometric analysis of rat splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Vermeer LA, de Boer NK, Bucci C, Bos NA, Kroese FG, Alberti S. 1994. MRC OX19 recognizes the rat CD5 surface glycoprotein, but does not provide evidence for a population of CD5bright B cells. Eur J Immunol. 24(3):585-92.

Vermeer L, Bos N, Kroese F, Bucci C, Alberti S. 1992. Molecular cloning of the rat CD5 gene. Ann N Y Acad Sci. 651:82-3.
de Boer NK, Ammerlaan WA, Meedendorp B, Kroese FG. 1992. CD5 B cells in the rat? Ann N Y Acad Sci. 651:157-9.

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