Product Information

Contents: Affinity Purified anti-mouse CD4 (L3T4)
Catalog Number: 14-0043
Sizes: 50 ug, 100 ug, 500 ug, 1 mg
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: RM4-4
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 thymocytes with .25 μg of Purified Rat IgG2b Isotype Control (cat. 14-4031) (open histogram) or .25 μg of Purified anti-mouse CD4 (RM4-4) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0043	Affinity Purified anti-mouse CD4 (L3T4)	N/A	N/A	FC
16-0043	Functional Grade* Purified anti-mouse CD4 (L3T4)	N/A	N/A	FC
13-0043	Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC
11-0043	Fluorescein isothiocyanate (FITC) anti-mouse CD4 (L3T4)	488	518	FC
12-0043	Phycoerythrin (PE) anti-mouse CD4 (L3T4)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RM4-4 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by the majority of thymocytes, a subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with the protein tyrosine kinase lck through its cytoplasmic tail. Binding of RM4-4 does not block binding of the CD4 monoclonal antibodies RM4-5 or GK1.5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RM4-4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This RM4-4 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Fredrickson, G.G., and R.S. Basch. 1989. L3T4 antigen expression by hemopoietic precursor cells. J. Exp. Med. 169: 1473 - 1478.

Wu, L., R. Scollay, M. Egerton, M. Pearse, G.J. Spangrude, and K. Shortman. 1991. CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus. Nature 349: 71 - 74.

Wu, L., M. Antica, G.R. Johnson, R. Scollay, and K. Shortman. 1991. Developmental potential of the earliest precursor cells from the adult mouse thymus. J. Exp. Med. 174: 1617 - 1627.

Godfrey, D.I., J. Kennedy, P. Mombaerts, S. Tonegawa, and A. Zlotnik. 1994. Onset of TCR-gene rearrangement and role of TCR-expression during CD3-CD4-CD8- thymocyte differentiation. J. Immunol. 152: 4783 - 4792.

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