Product Information

Contents: Affinity Purified anti-mouse CD4 (L3T4)
Catalog Number: 14-0042
Sizes: 50 ug, 100 ug, 500 ug, 1 mg
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: RM4-5
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified anti-mouse CD4 (RM4-5) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0042	FITC anti-mouse CD4 (L3T4)	488	518	FC
12-0042	PE anti-mouse CD4 (L3T4)	488	575	FC
13-0042	Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC
14-0042	Affinity Purified anti-mouse CD4 (L3T4)	N/A	N/A	FA FC IH/F
15-0042	PE-Cy5 anti-mouse CD4 (L3T4)	488	670	FC
16-0042	Functional Grade* Purified anti-mouse CD4 (L3T4)	N/A	N/A	FC
17-0042	APC anti-mouse CD4 (L3T4)	633	660	FC
25-0042	PE-Cy7 anti-mouse CD4 (L3T4)	488	760	FC
27-0042	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD4 (L3T4) (APC-Cy7 Equivalent)	633	779	FC
30-0042	DISCONTINUED - Allophycocyanin-Cy5.5 (APC-Cy5.5) anti-mouse CD4 (L3T4) (Alexa Fluor® 700 Equivalent)	633	690	FC
35-0042	PE-Cy5.5 anti-mouse CD4 (L3T4)	488	690	FC
45-0042	PerCP-Cy5.5 anti-mouse CD4 (L3T4)	488	690	FC
56-0042	Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent)	633	723	FC
57-0042	Pacific Blue® anti-mouse CD4 (L3T4)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RM4-5 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. It has also been reported in isolation and depletion of CD4⁺ cells and blocking of ligand binding. (Please use Functional Grade purified RM4-5, cat. 16-0042, in functional assays.)

Applications Tested

The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Okumura, K. 2000. Personal communication.
Wilde, D. B., P. Marrack, et al. 1983. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen- specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol 131(5): 2178-83.

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only.

Related Products

Cat. 11-0041    FITC anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 12-0041    PE anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 13-0041    Biotin anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 14-0041    Affinity Purified anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 15-0041    PE-Cy5 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 16-0041    Functional Grade Purified anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 17-0041    APC anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 19-0041    Cy5 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 25-0041    PE-Cy7 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4321    Affinity Purified Rat IgG2a Isotype Control
Cat. 11-4811    FITC Anti-Rat IgG
Cat. 13-4813    Biotin Anti-Rat IgG (clone Polyclonal)