Affinity Purified anti-mouse CD4 (L3T4)

RUO: For Research Use Only

mouse CD4 antibody, Affinity Purified conjugate

Staining of C57Bl/6 splenocytes with 0.25 μg of Purified Rat IgG2a Iso Cntrl (cat. 14-4321) (open histogram) or 0.25 μg of Purified anti-mouse CD4 (RM4-5) (colored histogram) followed by FITC Anti-Rat IgG (cat. 11-4811). Total viable cells were used for analysis.

Contents: Affinity Purified anti-mouse CD4 (L3T4)
Catalog Number: 14-0042
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Clone: RM4-5
Host/Isotype: Rat IgG2a, κ
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
14-0042-81	50 ug	To view relevant pricing, please select your country and resume browsing.
14-0042-82	100 ug
14-0042-85	500 ug
14-0042-86	1 mg

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-0041	FITC anti-mouse CD4 (L3T4) (GK1.5)
11-4317	Streptavidin-FITC (Fluorescein isothiocyanate)
11-4811	FITC Anti-Rat IgG
12-0041	PE anti-mouse CD4 (L3T4) (GK1.5)
12-4317	Streptavidin-PE (Phycoerythrin)
13-0041	Biotin anti-mouse CD4 (L3T4) (GK1.5)
13-4813	Biotin Anti-Rat IgG (Polyclonal)
14-0041	Affinity Purified anti-mouse CD4 (L3T4) (GK1.5)
14-4321	Affinity Purified Rat IgG2a Isotype Control
15-0041	PE-Cy5 anti-mouse CD4 (L3T4) (GK1.5)
16-0041	Functional Grade Purified anti-mouse CD4 (L3T4) (GK1.5)
17-0041	APC anti-mouse CD4 (L3T4) (GK1.5)
17-4317	Streptavidin Allophycocyanin (SA-APC)
19-0041	Cy5 anti-mouse CD4 (L3T4) (GK1.5)
25-0041	PE-Cy7 anti-mouse CD4 (L3T4) (GK1.5)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-0042	FITC anti-mouse CD4 (L3T4)	488	518	FC	RUO
12-0042	PE anti-mouse CD4 (L3T4)	488	575	FC	RUO
13-0042	Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC	RUO
14-0042	Affinity Purified anti-mouse CD4 (L3T4)	N/A	N/A	FA FC IH/F	RUO
15-0042	PE-Cy5 anti-mouse CD4 (L3T4)	488	670	FC	RUO
16-0042	Functional Grade* Purified anti-mouse CD4 (L3T4)	N/A	N/A	FC	RUO
17-0042	APC anti-mouse CD4 (L3T4)	633	660	FC	RUO
25-0042	PE-Cy7 anti-mouse CD4 (L3T4)	488	760	FC	RUO
45-0042	PerCP-Cy5.5 anti-mouse CD4 (L3T4) (PE-Cy5.5 replacement)	488	690	FC	RUO
46-0042	PerCP-eFluor® 710 anti-mouse CD4 (L3T4)	488	710	FC	RUO
47-0042	Allophycocyanin-eFluor® 780 anti-mouse CD4 (L3T4) (APC-Alexa Fluor® 750 replacement)	633	780	FC	RUO
48-0042	eFluor® 450 anti-mouse CD4 (L3T4) (Pacific Blue® replacement)	405	450	FC	RUO
56-0042	Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent)	633	723	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RM4-5 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. It has also been reported in isolation and depletion of CD4⁺ cells and blocking of ligand binding. (Please use Functional Grade purified RM4-5, cat. 16-0042, in functional assays.)

Applications Tested

The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Bliss SK, Bliss SP, Beiting DP, Alcaraz A, Appleton JA. IL-10 regulates movement of intestinally derived CD4+ T cells to the liver. J Immunol. 2007 Jun 15;178(12):7974-83. (RM4-5, FC, PubMed)

Rubinstein MP, Kovar M, Purton JF, Cho JH, Boyman O, Surh CD, Sprent J. Converting IL-15 to a superagonist by binding to soluble IL-15R{alpha}. Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9166-71. (RM4-5, FC, PubMed)

Irie J, Wu Y, Wicker LS, Rainbow D, Nalesnik MA, Hirsch R, Peterson LB, Leung PS, Cheng C, Mackay IR, Gershwin ME, Ridgway WM. NOD.c3c4 congenic mice develop autoimmune biliary disease that serologically and pathogenetically models human primary biliary cirrhosis. J Exp Med. 2006 May 15;203(5):1209-19. (RM4-5, IHC frozen, PubMed)

Andres PG, Beck PL, Mizoguchi E, Mizoguchi A, Bhan AK, Dawson T, Kuziel WA, Maeda N, MacDermott RP, Podolsky DK, Reinecker HC. Mice with a selective deletion of the CC chemokine receptors 5 or 2 are protected from dextran sodium sulfate-mediated colitis: lack of CC chemokine receptor 5 expression results in a NK1.1+ lymphocyte-associated Th2-type immune response in the intestine. J Immunol. 2000 Jun 15;164(12):6303-12. (RM4-5, IHC frozen)

Okumura, K. 2000.Personal communication.

Shi Y, Kaliyaperumal A, Lu L, Southwood S, Sette A, Michaels MA, Datta SK. Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain. J Exp Med. 1998 Feb 2;187(3):367-78. (RM4-5, FA)

Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83.