rat CD4 antibody, Affinity Purified conjugate

Contents: Affinity Purified anti-rat CD4
Catalog Number: 14-0040
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: OX35
Isotype: Mouse IgG2a

Product Options		Price
Cat. No.	Size	To view relevant pricing, please select your country and resume browsing.
14-0040-81	50 ug
14-0040-82	100 ug
14-0040-85	500 ug

Staining of rat splenocytes with FITC anti-rat CD3 (G4.18) (cat. 11-0030) and 0.25 μg of Purified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (left) or 0.25 μg of Purified anti-rat CD4 (OX35) (right) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (cat. 12-4012). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0040	Affinity Purified anti-rat CD4	N/A	N/A	FC IH/F
11-0040	Fluorescein isothiocyanate (FITC) anti-rat CD4	488	518	FC
17-0040	Allophycocyanin (APC) anti-rat CD4	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The OX35 monoclonal antibody reacts with rat CD4. CD4 is a co-receptor which participates in signaling through the T cell receptor by making contacts with MHC class II expressed on antigen-presenting cells (APC). CD4 is expressed in the thymus by CD4+CD8+ "double-positive" and CD4+ "single-positive" thymocytes, and by CD4+ "helper" T cells in the periphery. CD4 is a cell-surface membrane glycoprotein which contains four extracellular immunoglobulin-like domains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OX35 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.

Applications Tested

This OX35 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

May E, Dorris ML, Satumtira N, Iqbal I, Rehman MI, Lightfoot E, Taurog JD. CD8 alpha beta T cells are not essential to the pathogenesis of arthritis or colitis in HLA-B27 transgenic rats. J Immunol. 2003 Jan 15;170(2):1099-105. (OX35, FC, PubMed)

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