Product Information

Contents: Affinity Purified anti-human CD3
Catalog Number: 14-0039
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HIT3a
Isotype: Mouse IgG2a, κ
HLDA No.: V 5T-CD03.05

Profile of purified mAb HIT3a (CD3) staining on human peripheral blood lymphocytes and detection with FITC anti-mouse IgG (cat.no. 11-4011)

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0039	FITC anti-human CD3	488	518	FC
12-0039	PE anti-human CD3	488	575	FC
14-0039	Affinity Purified anti-human CD3	N/A	N/A	FC
16-0039	Functional Grade* Purified anti-human CD3	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HIT3a monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR with HIT3a initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIT3a antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HIT3a antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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