purified OKT3 antibody: anti-human CD3 antibody: purified OKT-3 antibody

Contents: Affinity Purified anti-human CD3
Catalog Number: 14-0037
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: OKT3
Isotype: Mouse IgG2a
HLDA No.: VI T6T-R3

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14-0037-80

25 ug

14-0037-82

100 ug

Staining of normal human peripheral blood cells with 0.125 µg of purified mouse IgG2a isotype control (cat.14-4724) (open histogram) or purified anti-human CD3 (clone OKT3) (colored histogram) followed by FITC anti-mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0037	Affinity Purified anti-human CD3	N/A	N/A	FC
16-0037	Functional Grade* Purified anti-human CD3	N/A	N/A	FA FC
13-0037	Biotin anti-human CD3	N/A	N/A	FC
11-0037	FITC anti-human CD3	488	518	FC
12-0037	PE anti-human CD3	488	575	FC
51-0037	Alexa Fluor® 647 anti-human CD3	633	668	FC
53-0037	Alexa Fluor® 488 anti-human CD3	488	519	FC
56-0037	Alexa Fluor® 700 anti-human CD3 (APC-Cy5.5 Equivalent)	633	723	FC
45-0037	PerCP-Cy5.5 anti-human CD3 (PE-Cy5.5 replacement)	488	690	FC
93-0037	eFluor^TM 605^NC anti-human CD3	350-500	605	FC
95-0037	eFluor^TM 650^NC anti-human CD3	350-500	650	FC
48-0037	eFluor^TM 450 anti-human CD3 (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, γ, δ, and ε chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The OKT3 antibody has been reported for use in flow cytometric analysis. OKT3 has also been reported in in vitro activation of T cells. (Please use Functional Grade purified OKT3, cat. 16-0037, in functional assays.)

Applications Tested

The OKT3 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hoffman RA et al. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980. PubMed: 81054881
Kung PC and Goldstein G. Complement-fixing monoclonal antibody to human T cells, and methods of preparing same. U.S. Pat. 4,361,549 dated Nov. 30, 1982.
Tanaka Y et al. Productive and lytic infection of human CD4+ type 1 helper T cells with macrophage-tropic human immunodeficiency virus type 1. J. Virol. 71: 465-470, 1997. PubMed: 97138346
Solomon KR et al. The association between glycoslphosphatidylinositol-anchored proteins and hetrotrimeric G protein alpha subunits in lymphocytes. Proc. Natl. Acad. Sci. USA 93: 6053-6058, 1996. PubMed: 96234088
Hutchcroft JE et al. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J. Biol. Chem. 271: 13362-13370, 1996. PubMed: 96278754
Adair, JR., et al. 1994. Humanization of the murine anti-human CD3 monoclonal antibody OKT3. Hum Antibodies Hybridomas. 5:41-7.

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