Product Information

Contents: Affinity Purified anti-mouse CD3 (CD3e, epsilon subunit)
Catalog Number: 14-0033
Sizes: 50 ug, 100 ug, 500 ug, 1 mg
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBio500A2 (500A2)
Isotype: Golden Syrian Hamster IgG

Staining of BALB/c splenocytes with 0.25 μg of Purified Golden Syrian Hamster IgG Iso Cntrl (cat. 14-4914) (open histogram) or 0.25 μg of Purified anti-mouse CD3e (500A2) (colored histogram) followed by FITC Anti-Syrian Hamster IgG (cat. 11-4211). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0033	Fluorescein isothiocyanate (FITC) anti-mouse CD3 (CD3e, epsilon subunit)	488	518	FC
12-0033	Phycoerythrin (PE) anti-mouse CD3 (CD3e, epsilon subunit)	488	575	FC
56-0033	Alexa Fluor® 700 anti-mouse CD3 (CD3e, epsilon subunit) (APC-Cy5.5 Equivalent)	633	723	FC
57-0033	Pacific Blue® anti-mouse CD3 (CD3e, epsilon subunit)	402	421	FC
14-0033	Affinity Purified anti-mouse CD3 (CD3e, epsilon subunit)	N/A	N/A	FA FC IH/F
16-0033	Functional Grade* Purified anti-mouse CD3 (CD3e, epsilon subunit)	N/A	N/A	FA FC
13-0033	Biotin anti-mouse CD3 (CD3e, epsilon subunit)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits γ, δ and ε are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eBio500A2 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in in vitro activation of mouse T cells. (Please use Functional Grade purified eBio500A2 in functional assays).

Applications Tested

This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Havran WL, Poenie M, Kimura J, Tsien R, Weiss A, Allison JP. Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes. Nature. 1987 Nov 12-18;330(6144):170-3. (500A2, FC, FA, PubMed)

Portoles P, Rojo J, Golby A, Bonneville M, Gromkowski S, Greenbaum L, Janeway CA Jr, Murphy DB, Bottomly K. Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation. J Immunol. 1989 Jun 15;142(12):4169-75. (PubMed)

Ortaldo JR, Winkler-Pickett R, Mason AT, Mason LH. The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells. J Immunol. 1998 Feb 1;160(3):1158-65. (500A2, FC, PubMed)

Related Products

Cat. 14-0031    Affinity Purified Hamster anti-mouse CD3 (epsilon subunit; CD3e) (clone 145-2C11)
Cat. 11-4211    FITC Anti-Syrian Hamster IgG (clone Polyclonal)
Cat. 13-4213    Biotin Anti-Syrian Hamster IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4914    Affinity Purified Golden Syrian Hamster IgG Isotype Control (clone n/a)