Product Information

Contents: Affinity Purified anti-mouse CD3
Catalog Number: 14-0032
Sizes: 50 ug, 100 ug, 500 ug, 1 mg
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 17A2
Isotype: Rat IgG2b, κ

Surface staining of mouse thymocytes with purified anti-mouse CD3 (17A2) followed by anti-rat IgG FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0032	Affinity Purified anti-mouse CD3 (CD3e, epsilon subunit)	N/A	N/A	FA FC IH/F
16-0032	Functional Grade* Purified anti-mouse CD3 (CD3e, epsilon subunit)	N/A	N/A	FA FC
51-0032	Alexa Fluor® 647 anti-mouse CD3 (CD3e, epsilon subunit)	633	668	FC
56-0032	Alexa Fluor® 700 anti-mouse CD3 (CD3e, epsilon subunit) (APC-Cy5.5 Equivalent)	633	723	FC
47-0032	Allophycocyanin-eFluor^TM 780 anti-mouse CD3 (CD3e, epsilon subunit) (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0032	eFluor^TM 450 anti-mouse CD3 (CD3e, epsilon subunit) (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits γ, δ and ε are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 17A2 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro activation of mouse T cells. (Please use Functional Grade purified 17A2 in functional assays).

Applications Tested

This 17A2 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Miescher, G. C., M. Schreyer, et al. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989: 23(2): 113-8.

Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC. Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol. 2002 Mar 15;168(6):3088-98.(17A2, IH/F, PubMed)

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