mouse CD3 antibody: purified antibody

Contents: Affinity Purified anti-mouse CD3
Catalog Number: 14-0032
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 17A2
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-0032-81	50 ug	$50
14-0032-82	100 ug	$70
14-0032-85	500 ug	$180
14-0032-86	1 mg	$320

*International customers: Please contact your distributor for region specific pricing.

Surface staining of mouse thymocytes with purified anti-mouse CD3 (17A2) followed by anti-rat IgG FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0032	Affinity Purified Rat anti-mouse CD3 (CD3e, epsilon subunit)	N/A	N/A	FA FC IH/F
16-0032	Functional Grade* Purified Rat anti-mouse CD3 (CD3e, epsilon subunit)	N/A	N/A	FA FC
51-0032	Alexa Fluor® 647 anti-mouse CD3 (CD3e, epsilon subunit)	633	668	FC
56-0032	Alexa Fluor® 700 anti-mouse CD3 (CD3e, epsilon subunit) (APC-Cy5.5 Equivalent)	633	723	FC
57-0032	Pacific Blue® anti-mouse CD3 (CD3e, epsilon subunit)	402	421	FC
27-0032	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD3 (CD3e, epsilon subunit) (APC-Cy7 Equivalent)	633	779	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits γ, δ and ε are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 17A2 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro activation of mouse T cells. (Please use Functional Grade purified 17A2 in functional assays).

Applications Tested

This 17A2 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Miescher, G. C., M. Schreyer, et al. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989: 23(2): 113-8.

Zhang Y, McCormick LL, Desai SR, Wu C, Gilliam AC. Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation. J Immunol. 2002 Mar 15;168(6):3088-98.(17A2, IH/F, PubMed)

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