Product Information

Contents: Affinity Purified anti-human CD1a
Catalog Number: 14-0019
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: HI149
Isotype: Mouse IgG1, κ
HLDA No.: V 5T CD01.01

Staining of MOLT-4 cells with 0.5 μg affinity purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or affinity purified anti-human CD1a (HI149) (colored histogram). Total viable cells were used for the analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0019	FITC anti-human CD1a	488	518	FC
12-0019	PE anti-human CD1a	488	575	FC
13-0019	Biotin anti-human CD1a	N/A	N/A	FC
14-0019	Affinity Purified anti-human CD1a	N/A	N/A	FC IHC
51-0019	Alexa Fluor® 647 anti-human CD1a	633	668	FC
53-0019	Alexa Fluor® 488 anti-human CD1a	488	519	FC
57-0019	Pacific Blue® anti-human CD1a	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HI149 monoclonal antibody reacts with human CD1a, a 49 kDa protein expressed by cortical thymocytes and dendritic cells including Langerhans cells. The CD1 family of proteins share some structural and functional characteristics with the MHC class I molecules; however, members of the CD1 family are not polymorphic. Similar to MHC class I, CD1a associates with the β2-microglobulin and is thought to play a role in antigen presentation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HI149 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested

This HI149 antibody has been tested by flow cytometric analysis of human MOLT-4 cell line. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control