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Human Cellular Antigens Chart

Contents: Affinity Purified anti-human CD1a Catalog Number: 14-0019 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: HI149 Isotype: Mouse IgG1, κ HLDA No.: V 5T CD01.01 Prices for This Product* Cat. No. Size Price Qty Action 14-0019-80 25 ug $50 14-0019-82 100 ug $95 *International customers: Please contact your distributor for region specific pricing.

Staining of MOLT-4 cells with 0.5 μg affinity purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or affinity purified anti-human CD1a (HI149) (colored histogram). Total viable cells were used for the analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0019	Affinity Purified anti-human CD1a	N/A	N/A	FC  IHC
13-0019	Biotin anti-human CD1a	N/A	N/A	FC
11-0019	FITC anti-human CD1a	488	518	FC
12-0019	PE anti-human CD1a	488	575	FC
51-0019	Alexa Fluor® 647 anti-human CD1a	633	668	FC
53-0019	Alexa Fluor® 488 anti-human CD1a	488	519	FC
57-0019	Pacific Blue® anti-human CD1a	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The HI149 monoclonal antibody reacts with human CD1a, a 49 kDa protein expressed by cortical thymocytes and dendritic cells including Langerhans cells. The CD1 family of proteins share some structural and functional characteristics with the MHC class I molecules; however, members of the CD1 family are not polymorphic. Similar to MHC class I, CD1a associates with the β2-microglobulin and is thought to play a role in antigen presentation.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The HI149 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

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Applications Tested

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This HI149 antibody has been tested by flow cytometric analysis of human MOLT-4 cell line. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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Related Products

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Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714    Affinity Purified Mouse IgG1, K Isotype Control