Product Information

Contents: Affinity Purified anti-human CD1b
Catalog Number: 14-0018
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioSN13 (SN13 K5-1B8)
Isotype: Mouse IgG1

Staining of Molt-4 cells with 0.5 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.5 μg of Purified anti-human CD1b (SN13) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013)and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0018	Affinity Purified anti-human CD1b	N/A	N/A	FC IH/F IP
51-0018	Alexa Fluor® 647 anti-human CD1b	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioSN13 monoclonal antibody reacts with human CD1b. The CD1 molecules are similar to MHC class I, but rather than presenting peptide antigens to T-cell receptors (TCR), they present a diversity of lipid and glycolipid antigens which are derived from self as well as from mycobacteria which include sphingolipids, mycolic acid derivatives and phosphatidylinositols such as liparabinomannan (LAM) and glucose monomycolate (GMM). CD1b is 45 kDa, and like the other CD1 antigens, is expressed as a heterodimer composed of the CD1b noncovalently paired with β2-microglobulin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioSN13 (SN13 K5-1B8) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.

Applications Tested

This eBioSN13 (SN13 K5-1B8) antibody has been tested by flow cytometric analysis of SN13 cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Martin LH, Calabi F, Lefebvre FA, Bilsland CA, Milstein C. Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9189-93.

Porcelli S, Morita CT, Brenner MB. CD1b restricts the response of human CD4-8- T lymphocytes to a microbial antigen. Nature. 1992 Dec 10;360(6404):593-7.

Giuliani A, Prete SP, Graziani G, Aquino A, Balduzzi A, Sugita M, Brenner MB, Iona E, Fattorini L, Orefici G, Porcelli SA, Bonmassar E. Influence of Mycobacterium bovis bacillus Calmette Guérin on in vitro induction of CD1 molecules in human adherent mononuclear cells. Infect Immun. 2001 Dec;69(12):7461-70. (SN13, FC, PubMed)

Related Products

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Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control