Affinity Purified anti-human CD1d

Contents: Affinity Purified anti-human CD1d
Catalog Number: 14-0016
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 51.1
Isotype: Mouse IgG2b

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-0016-80	25 ug	$50
14-0016-82	100 ug	$95

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with 0.5 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (open histogram) or Purified anti-human CD1d (51.1) (colored histogram) followed by Biotin Anti-Mouse IgG (cat. 13-4013) and Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0016	Affinity Purified anti-human CD1d	N/A	N/A	FC IH/F IP
12-0016	Phycoerythrin (PE) anti-human CD1d	488	575	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody 51.1 reacts with human CD1d, a member of the CD1 family with similarity to the non-polymorphic MHC Class I-like molecules. CD1d is a highly conserved single transmembrane receptor of the Immunoglobulin Superfamily. CD1d can associate with β-microglobulin another feature showing similarity to MHC class I molecules, but can also exist as a nonglycosylated protein not in association with β microglobulin. This suggests different control mechanisms for presenting glycolipid containing molecules to CD1d reactive NKT cells. Expression of CD1d is found on B cells of the periphery, in resting monocytes and cortical thymocytes. On intestinal epithelial cells (IEC) expression is polarized. Expression can also be found at low levels intracellularly in hepatocytes. In HCV (hepatitis C virus) livers, CD1d is highly expressed compared to normal controls.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 51.1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.

Applications Tested

This 51.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Blumberg RS, Terhorst C, Bleicher P, McDermott FV, Allan CH, Landau SB, Trier JS, Balk SP. Expression of a nonpolymorphic MHC class I-like molecule, CD1D, by human intestinal epithelial cells. J Immunol. 1991 Oct 15;147(8):2518-24.

Durante-Mangoni E, Wang R, Shaulov A, He Q, Nasser I, Afdhal N, Koziel MJ, Exley MA. Hepatic CD1d expression in hepatitis C virus infection and recognition by resident proinflammatory CD1d-reactive T cells. J Immunol. 2004 Aug 1;173(3):2159-66. (51.1 IHf)

Exley M, Garcia J, Wilson SB, Spada F, Gerdes D, Tahir SM, Patton KT, Blumberg RS, Porcelli S, Chott A, Balk SP. CD1d structure and regulation on human thymocytes, peripheral blood T cells, B cells and monocytes. Immunology. 2000 May;100(1):37-47.

Somnay-Wadgaonkar K, Nusrat A, Kim HS, Canchis WP, Balk SP, Colgan SP, Blumberg RS. Immunolocalization of CD1d in human intestinal epithelial cells and identification of a beta2-microglobulin-associated form. Int Immunol. 1999 Mar;11(3):383-92

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