Product Information

Contents: Biotin anti-human Ig light chain κ
Catalog Number: 13-9997
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HP6062
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9997	Affinity Purified anti-human IgL, kappa	N/A	N/A	ELISA cap FC IH/F
13-9997	Biotin anti-human IgL, kappa	N/A	N/A	ELISA det FC IH/F
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HP6062 monoclonal antibody is specific for the κ light chain of human immunoglobulin. It does not react with the λ light chain. Ig light chain is expressed as a membrane-bound form as well as soluble form.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HP6062 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA.

Applications Tested

The HP6062 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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