Product Information

Contents: Biotin anti-human Ig light chain λ
Catalog Number: 13-9996
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: JDC-12
Isotype: Mouse IgG1, κ

Surface staining of normal human peripheral blood cells with anti-human IgL, λ (JDC-12) Purified followed by anti-mouse IgG Biotin and SA-PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9996	Affinity Purified anti-human IgL, lambda	N/A	N/A	ELISA cap FC IH/F
13-9996	Biotin anti-human IgL, lambda	N/A	N/A	ELISA det FC IH/F
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The JDC-12 monoclonal antibody reacts with the λ light chain of human immunoglobulin. It does not react with the κ light chain. Ig light chain is expressed as a membrane-bound form as well as soluble form.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The JDC-12 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA.

Applications Tested

The JDC-12 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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