Product Information

Contents: Biotin anti-mouse PD-1 (CD279, PD1)
Catalog Number: 13-9985
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: J43
Isotype: Armenian Hamster IgG

Surface staining of ConA stimulated splenocytes with anti-mouse PD-1 (J43) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9985	Affinity Purified anti-mouse PD-1 (CD279, PD1)	N/A	N/A	FA FC IH/F IP
16-9985	Functional Grade* Purified anti-mouse PD-1 (CD279, PD1)	N/A	N/A	FC
13-9985	Biotin anti-mouse PD-1 (CD279, PD1)	N/A	N/A	FC
11-9985	FITC anti-mouse PD-1 (CD279, PD1)	488	518	FC
12-9985	PE anti-mouse PD-1 (CD279, PD1)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The J43 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Agata Y, Kawasaki A, et al. (1996). Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes. Int Immunol 8(5): 765-72.

Ansari M, Salama AD, et al. 2003. The Programmed Death-1 (PD-1) Pathway Regulates Autoimmune Diabetes in Nonobese Diabetic (NOD) Mice. JEM. 198:63-69 (IH/F, FA, PubMed).

Nishimura H, Agata Y, et al. (1996). Developmentally regulated expression of the PD-1 protein on the surface of double-negative (CD4-CD8-) thymocytes. Int Immunol 8(5): 773-80.

Nishimura, H., T. Okazaki, et al. (2001). Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice. Science 291(5502): 319-22.

Freeman, G. J., A. J. Long, et al. (2000). Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med 192(7): 1027-34.

Nishimura, H., T. Honjo, et al. (2000). Facilitation of beta selection and modification of positive selection in the thymus of PD-1-deficient mice. J Exp Med 191(5): 891-8.

Nishimura, H., N. Minato, et al. (1998). Immunological studies on PD-1 deficient mice: implication of PD-1 as a negative regulator for B cell responses. Int Immunol 10(10): 1563-72.

Salama AD, Chitnis T, Imitola J, Ansari MJ, Akiba H, Tushima F, Azuma M, Yagita H, Sayegh MH, Khoury SJ. Critical role of the programmed death-1 (PD-1) pathway in regulation of experimental autoimmune encephalomyelitis. J Exp Med. 2003 Jul 7;198(1):71-8. (IH/F, FA, PubMed)

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