mouse AID antibody, Biotin conjugate

Contents: Biotin anti-mouse AID (Activation-induced cytidine deaminase)
Catalog Number: 13-9974
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBio961
Isotype: Rat IgG2a, κ

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13-9974-80

25 ug

13-9974-82

100 ug

Staining of mouse AID-transfected 293T cells with 0.5 μg of Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (open histogram) or 0.25 μg of Biotin anti-mouse AID (eBio961) (colored histogram) followed by SAv-APC (cat. 17-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9974	Affinity Purified anti-mouse AID (Activation-induced cytidine deaminase)	N/A	N/A	WB
13-9974	Biotin anti-mouse AID (Activation-induced cytidine deaminase)	N/A	N/A	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio961 antibody reacts with mouse activation-induced cytidine deaminase (AID), a member of the cytidine deaminase family, which was cloned from a mouse B-cell lymphoma line induced by combined stimulation with transforming growth factor-beta, interleukin-4, and CD40 ligand. The human AID gene encodes a 198-amino acid protein containing a conserved cytidine deaminase motif. The human AID protein shares 92% amino acid identity with mouse AID. RT-PCR analysis of 15 human tissues detected strong expression of AID mRNA in lymph nodes and tonsils.
Class switch recombination (CSR) is a region-specific DNA recombination reaction that replaces one immunoglobulin (Ig) heavy-chain constant region gene with another. This enables a single variable region gene to be used in conjunction with different downstream heavy-chain constant region genes, each having a unique biologic activity. AID, a putative RNA editing enzyme, is required for this action.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio961 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio961 antibody has been tested by flow cytometric analysis of mouse AID-transfected 293T cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Muramatsu, M., V.S. Sankaranand, S. Anant, M. Sugai, K. Kinoshita, N.O. Davidson, and T. Honjo. 1999. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem. 274: 18470-18476.

Kinoshita, K., M. Harigai, S. Fagarasan, M. Muramatsu, and T. Honjo. 2001. A hallmark of active class switch recombination: transcripts directed by I promoters on looped-out circular DNAs. PNAS. 98: 12620-12633.

Nagaoka H., M. Muramatsu, N. Yamamura, K. Kinoshita, and T. Honjo. 2002. Activation-induced deaminase (AID)-directed hypermutation in the immunoglobulin Sµ region: implication of AID involvement in a common step of class switch recombination and somatic hypermutation. J. Exp. Med. 195: 539-534.

Related Products

Cat. 13-4321  Biotin Rat IgG2a Isotype Control
Cat. 11-9984  Fluorescein isothiocyanate (FITC) anti-mouse AID (Activation-induced cytidine deaminase) (clone eBio911)
Cat. 14-9984  Affinity Purified anti-mouse AID (Activation-induced cytidine deaminase) (clone eBio911)