mouse B7-DC antibody, Biotin conjugate

Contents: Biotin anti-mouse B7-DC (B7DC, PD-L2, PDL-2, PDL2)
Catalog Number: 13-9972
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 122
Isotype: Rat IgG2a, κ

Product Options		Price
Cat. No.	Size	To view relevant pricing, please select your country and resume browsing.
13-9972-81	50 ug
13-9972-82	100 ug
13-9972-85	500 ug

Staining of in vitro BM-derived dendritic cells from BALB/c with 0.06 μg Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (open histogram) or 0.06 μg Biotin anti-mouse B7-DC (122)(colored histogram) followed by Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9972	Affinity Purified anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2)	N/A	N/A	FC
13-9972	Biotin anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2)	N/A	N/A	FC
11-9972	Fluorescein (FITC) anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2)	488	518	FC
12-9972	Phycoerythrin (PE) anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 122 monoclonal antibody reacts with mouse B7-DC, also known as PD-L2. B7-DC, a recently identified member of the B7 family, has a predicted molecular weight of approximately 25 kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by subpopulations of dendritic cells and monocytes/macrophages in the mouse. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, rather it is a ligand for PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. 122 has been demonstrated to block binding of TY25 (Cat. No. 14-5986), another mAb specific for mouse B7-DC.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 122 has been reported for use in flow cytometric analysis.

Applications Tested

The 122 antibody has been tested by flow cytometric analysis of mouse dendritic cell suspensions and mouse B7-DC transfected cells. This can be used at less than or equal to 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ishida M, Iwai Y, Tanaka Y, Okazaki T, Freeman GJ, Minato N, Honjo T. 2002. Differential expression of PD-L1 and PD-L2, ligands for an inhibitory receptor PD-1, in the cells of lymphohematopoietic tissues. Immunol Lett. 84(1):57-62. Carter L, Fouser LA, Jussif J, Fitz L, Deng B, Wood CR, Collins M, Honjo T, Freeman GJ, Carreno BM. 2002. PD-1:PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2. Eur J Immunol. 32(3):634-43.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4321  Biotin Rat IgG2a Isotype Control
Cat. 12-5986  PE anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone TY25)
Cat. 13-5986  Biotin anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone TY25)
Cat. 14-5986  Affinity Purified anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone TY25)
Cat. 16-5986  Functional Grade Purified anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone TY25)