Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e APC (cat. 17-0031) and 0.06 µg of Rat IgG2a Isotype Control Biotin (cat. 13-4321) (left) or 0.06 µg of Anti-Mouse CD274 (B7-H1) Biotin (right) followed by Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.
Contents: Anti-Mouse CD274 (B7-H1) Biotin Catalog Number: 13-9971 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Clone: 1-111A Host/Isotype: Rat IgG2a, κ
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Cat. No.
Size
Price
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13-9971-81
50 ug
13-9971-82
100 ug
13-9971-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 1-111A monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. Preliminary testing suggests that 1-111A and MIH5 (Cat. No. 14-5982) cross-block each other.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 1-111A antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 1-111A antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ishida M, Iwai Y, Tanaka Y, Okazaki T, Freeman GJ, Minato N, Honjo T. 2002. Differential expression of PD-L1 and PD-L2, ligands for an inhibitory receptor PD-1, in the cells of lymphohematopoietic tissues. Immunol Lett. 84(1):57-62. Carter L, Fouser LA, Jussif J, Fitz L, Deng B, Wood CR, Collins M, Honjo T, Freeman GJ, Carreno BM. 2002. PD-1:PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2. Eur J Immunol. 32(3):634-43.
