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Biotin anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)

 
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Contents: Biotin anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)
Catalog Number: 13-9971
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 1-111A
Isotype: Rat IgG2a, κ

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13-9971-81 50 ug
13-9971-82 100 ug
13-9971-85 500 ug


   
data image 1 data image 2
Staining of C57Bl/6 splenocytes with APC anti-mouse CD3e (145-2C11) (cat. 17-0031) and 0.06 μg Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (left) or 0.06 μg Biotin anti-mouse B7-H1 (1-111A)(right) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.
Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
14-9971 Affinity Purified anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1) N/A N/A FC 
13-9971 Biotin anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1) N/A N/A FC 
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description


The 1-111A monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. Preliminary testing suggests that 1-111A and MIH5 (Cat. No. 14-5982) cross-block each other.


Applications Reported


For research use only, not for diagnostic or therapeutic use. The 1-111A antibody has been reported for use in flow cytometric analysis.


Applications Tested


The 1-111A antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.


References


Ishida M, Iwai Y, Tanaka Y, Okazaki T, Freeman GJ, Minato N, Honjo T. 2002. Differential expression of PD-L1 and PD-L2, ligands for an inhibitory receptor PD-1, in the cells of lymphohematopoietic tissues. Immunol Lett. 84(1):57-62. Carter L, Fouser LA, Jussif J, Fitz L, Deng B, Wood CR, Collins M, Honjo T, Freeman GJ, Carreno BM. 2002. PD-1:PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2. Eur J Immunol. 32(3):634-43.


Related Products


Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4321  Biotin Rat IgG2a Isotype Control
Cat. 12-5982  PE anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1) (clone MIH5)
Cat. 13-5982  Biotin anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1) (clone MIH5)
Cat. 14-5982  Affinity Purified anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1) (clone MIH5)
Cat. 16-5982  Functional Grade Purified anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1) (clone MIH5)
Cat. 12-5984  PE anti-mouse B7-H1 (B7H1, PD-L1, PDL-1, PDL1) (clone MIH6)
Cat. 13-5984  Biotin anti-mouse B7-H1 (B7H1, PD-L1, PDL-1, PDL1) (clone MIH6)
Cat. 14-5984  Affinity Purified anti-mouse B7-H1 (B7H1, PD-L1, PDL-1, PDL1) (clone MIH6)


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