Biotin anti-human TRAIL (CD253, Apo-2L) antibody: biotin conjugate

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Contents: Biotin anti-human Trail (CD253)
Catalog Number: 13-9927
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: RIK-2
Isotype: Mouse IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
13-9927-80	25 ug	$95
13-9927-82	100 ug	$220

*International customers: Please contact your distributor for region specific pricing.

Staining of Biotin anti-human TRAIL (RIK-2) on human TRAIL transfected cells (detected with Streptavidin-PE)

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-9927	PE anti-human Trail (CD253)	488	575	FC
14-9927	Affinity Purified anti-human Trail (CD253)	N/A	N/A	FA FC
16-9927	Functional Grade* Purified anti-human Trail (CD253)	N/A	N/A	FA FC
13-9927	Biotin anti-human Trail (CD253)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RIK-2 monoclonal antibody reacts with human TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is expressed by normal human T, B and NK cells and by several human tumor lines. Interaction of TRAIL with its ligand, Apo-2, induces apoptosis. RIK-2 blocks TRAIL-induced apoptosis.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RIK-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The RIK-2 antibody has been tested by flow cytometric analysis of human TRAIL transfected cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kayagaki, N., N. Yamaguchi, et al. (1999). "Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells." J Immunol 163(4): 1906-13.
Kaplan, M. J., D. Ray, et al. (2000). "TRAIL (Apo2 ligand) and TWEAK (Apo3 ligand) mediate CD4+ T cell killing of antigen-presenting macrophages [In Process Citation]." J Immunol 164(6): 2897-904.
Kayagaki, N., N. Yamaguchi, et al. (1999). "Involvement of TNF-related apoptosis-inducing ligand in human CD4+ T cell-mediated cytotoxicity." J Immunol 162(5): 2639-47.

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