Product Information

Contents: Biotin anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)
Catalog Number: 13-9922
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: TL2.1
Isotype: Mouse IgG2a, κ

TL2.1 Biotin + SA-PE vs. Mouse IgG2a Biotin Isotype Control (Gated on normal peripheral monocytes)

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9922	Affinity Purified anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC IHC IP WB
16-9922	Functional Grade* Purified anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FA FC
13-9922	Biotin anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC
11-9922	FITC anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	518	FC
12-9922	PE anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	575	FC
17-9922	Allophycocyanin (APC) anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	633	660	FC
51-9922	Alexa Fluor® 647 anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	633	668	FC
53-9922	Alexa Fluor® 488 anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	519	FC
56-9922	Alexa Fluor® 700 anti-human Toll-like receptor 2 (CD282, TLR2, TLR-2) (APC-Cy5.5 Equivalent)	633	723	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The TL2.1 monoclonal antibody reacts with human Toll-like receptor 2 (TLR2). To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed by peripheral blood monocytes and is responsible for distinguishing different pathogens. TL2.1, a blocking antibody, has been used to study the role of TLR-2 as a pattern recognition receptor in microbial lipoprotein/lipopeptide induced cytokine production from human peripheral blood mononuclear cells. TL2.1 has been reported to immunoprecipitate human TLR2 (~90 kDa) from PBMC and HMEC.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The TL2.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The TL2.1 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008 Jul 15;124(1-2):184-91. (TL2.1, canine cross-reactivity, PubMed)

Do JE, Kwon SY, Park S, Lee ES. Effects of vitamin D on expression of Toll-like receptors of monocytes from patients with Behcet's disease. Rheumatology (Oxford). 2008 Jun;47(6):840-8. (TL2.1, FA, PubMed)

Mandron M, Ariès MF, Boralevi F, Martin H, Charveron M, Taieb A, Davrinche C. Age-related differences in sensitivity of peripheral blood monocytes to lipopolysaccharide and Staphylococcus aureus toxin B in atopic dermatitis. J Invest Dermatol. 2008 Apr;128(4):882-9. (TL2.1, FA, PubMed)

Bonnefont-Rebeix C, Marchal T, Bernaud J, Pin JJ, Leroux C, Lebecque S, Chabanne L, Rigal D. Toll-like receptor 3 (TLR3): a new marker of canine monocytes-derived dendritic cells (cMo-DC). Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):134-9. (TL2.1, canine cross-reactivity, PubMed)

Chan G, Guilbert LJ. Ultraviolet-inactivated human cytomegalovirus induces placental syncytiotrophoblast apoptosis in a Toll-like receptor-2 and tumour necrosis factor-alpha dependent manner. J Pathol. 2006 Sep;210(1):111-20. (TL2.1, WB and IHC frozen, PubMed)

Cook EB, Stahl JL, Esnault S, Barney NP, Graziano FM. Toll-like receptor 2 expression on human conjunctival epithelial cells: a pathway for Staphylococcus aureus involvement in chronic ocular proinflammatory responses. Ann Allergy Asthma Immunol. 2005 Apr;94(4):486-97.(TL2.1, FA, PubMed)

Ueta M, Nochi T, Jang MH, Park EJ, Igarashi O, Hino A, Kawasaki S, Shikina T, Hiroi T, Kinoshita S, Kiyono H. Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium. J Immunol. 2004 Sep 1;173(5):3337-47. (TL2.1, IHC frozen, PubMed)

Iwahashi M, Yamamura M, Aita T, Okamoto A, Ueno A, Ogawa N, Akashi S, Miyake K, Godowski PJ, Makino H. Expression of Toll-like receptor 2 on CD16+ blood monocytes and synovial tissue macrophages in rheumatoid arthritis. Arthritis Rheum. 2004 May;50(5):1457-67. (TL2.1, FA, PubMed)

Coleman JL, Benach JL. The urokinase receptor can be induced by Borrelia burgdorferi through receptors of the innate immune system. Infect Immun. 2003 Oct;71(10):5556-64. (TL2.1, FA, PubMed)

Mori Y, Yoshimura A, Ukai T, Lien E, Espevik T, Hara Y. Immunohistochemical localization of Toll-like receptors 2 and 4 in gingival tissue from patients with periodontitis. Oral Microbiol Immunol. 2003 Feb;18(1):54-8. (TL2.1, IHC paraffin, PubMed)

Droemann D, Goldmann T, Branscheid D, Clark R, Dalhoff K, Zabel P, Vollmer E. Toll-like receptor 2 is expressed by alveolar epithelial cells type II and macrophages in the human lung. Histochem Cell Biol. 2003 Feb;119(2):103-8. (TL2.1, IHC paraffin, PubMed)

Faure E, Thomas L, Xu H, Medvedev A, Equils O, Arditi M. Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappaB activation. J Immunol. 2001 Feb 1;166(3):2018-24. (TL2.1, WB and IHC frozen, PubMed)

Flo TH, Halaas O, Lien E, Ryan L, Teti G, Golenbock DT, Sundan A, Espevik T. Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol. 2000 Feb 15;164(4):2064-9. (TL2.1, Immunoprecipitation)

Lien E, Sellati TJ, Yoshimura A, Flo TH, Rawadi G, Finberg RW, Carroll JD, Espevik T, Ingalls RR, Radolf JD, Golenbock DT. Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products. J Biol Chem. 1999 Nov 19;274(47):33419-25.

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Pat. No. US 7,071,310