human TWEAK antibody, Biotin conjugate

Contents: Biotin anti-human TWEAK
Catalog Number: 13-9915
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: CARL-1
Isotype: Mouse IgG3, κ

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13-9915-80

25 ug

13-9915-82

100 ug

Staining of human TWEAK-transfected cells with 0.5 μg of Iso Cntrl (open histogram) or 0.5 μg of Biotin anti-human TWEAK (CARL-1) (colored histogram) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9915	Affinity Purified anti-human TWEAK	N/A	N/A	FA FC
16-9915	Functional Grade* Purified anti-human TWEAK	N/A	N/A	FA FC
13-9915	Biotin anti-human TWEAK	N/A	N/A	ELISA det FC
12-9915	PE anti-human TWEAK	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The CARL-1 monoclonal antibody reacts with human TWEAK, a type II transmembrane TNF superfamily member with high identity to TNF in its extracellular portion. TWEAK transcript is expressed broadly in many adult and fetal tissues, however, the staining of human peripheral blood mononuclear cells with monoclonal antibodies shows a more restricted pattern. While freshly isolated PBMCs do not express detectable levels of TWEAK on their surface, IFN-γ-stimulated blood monocytes rapidly upregulate TWEAK surface expression. TWEAK is expressed as membrane bound and secreted forms. Interaction of TWEAK with its counter-receptor promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, and apoptosis in a number of human cell lines. Initially, DR3 was thought to be a receptor for TWEAK, but further studies have shown that TWEAK could induce apoptosis via receptors distinct from DR3. While TWEAK exhibits overlapping signaling functions to TNF, it is generally less effective in inducing apoptosis, giving rise to its name, TNF-like weak inducer of apoptosis. For detection of human TWEAK by sandwich ELISA, a combination of purified CARL-2 for capture and biotinylated CARL-1 for detection is recommended.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CARL-1 antibody has been reported for use in flow cytometric analysis, and ELISA.

Applications Tested

The CARL-1 antibody has been tested by flow cytometric analysis of transfected cells and IFN-γ-stimulated human PBMCs. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nakayama, M., N. Kayagaki, et al. (2000). "Involvement of TWEAK in Interferon {gamma}-stimulated Monocyte Cytotoxicity." J. Exp. Med. 192(9): 1373-1380.
Chicheportiche, Y., P. R. Bourdon, et al. (1997). "TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis." J Biol Chem 272(51): 32401-10.
Kaplan, M. J., D. Ray, et al. (2000). "TRAIL (Apo2 ligand) and TWEAK (Apo3 ligand) mediate CD4+ T cell killing of antigen-presenting macrophages [In Process Citation]." J Immunol 164(6): 2897-904.
Lynch, C. N., Y. C. Wang, et al. (1999). "TWEAK induces angiogenesis and proliferation of endothelial cells." J Biol Chem 274(13): 8455-9.
Schneider, P., R. Schwenzer, et al. (1999). "TWEAK can induce cell death via endogenous TNF and TNF receptor 1." Eur J Immunol 29(6): 1785-92.

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