Product Information

Contents: Biotin anti-human DR5 (CD262, Trail-R2, TrailR2)
Catalog Number: 13-9908
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: DJR2-4 (a.k.a. 7-8)
Isotype: Mouse IgG1, κ

Surface staining of human DR5 transfected cells with anti-human DR5 (DJR2-4 ) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9908	Affinity Purified anti-human DR5 (CD262, Trail-R2, TrailR2)	N/A	N/A	FC
13-9908	Biotin anti-human DR5 (CD262, Trail-R2, TrailR2)	N/A	N/A	FC
12-9908	PE anti-human DR5 (CD262, Trail-R2, TrailR2)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The DJR2-4 monoclonal antibody reacts with human DR5, also known as TRAIL-R2, Apo2, TRICK2 and KILLER. DR5 binds to TRAIL, activates NF-κB, and induces TRAIL-mediated apoptosis. DR5 is expressed broadly by normal tissues as well as several tumor cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DJR2-4 (a.k.a. 7-8) antibody has been reported for use in flow cytometric analysis.

Applications Tested

The DJR2-4 (a.k.a. 7-8) antibody has been tested by flow cytometric analysis of human MOLT-4 cell line and human DR5-transfected cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Earel JK Jr, Vanoosten RL, Griffith TS. Histone deacetylase inhibitors modulate the sensitivity of tumor necrosis factor-related apoptosis-inducing ligand-resistant bladder tumor cells. Cancer Res. 2006 Jan 1;66(1):499-507. (FC, PubMed)

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