Product Information

Contents: Biotin anti-mouse Toll-like receptor 9 (CD289, TLR9, TLR-9)
Catalog Number: 13-9093
Sizes: 25 ug, 50 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: M9.D6
Isotype: Rat IgG2a

Staining of fixed and permeabilized mouse TLR9-transfected 293 cells with 0.25 μg Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (open histogram) or 0.25 μg Biotin anti-mouse TLR9 (M9.D6)(colored histogram) followed by Streptavidin-PE (cat. 12-4317). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9093	Affinity Purified anti-mouse Toll-like receptor 9 (CD289, TLR9, TLR-9)	N/A	N/A	IC Flow WB
13-9093	Biotin anti-mouse Toll-like receptor 9 (CD289, TLR9, TLR-9)	N/A	N/A	IC Flow WB
11-9093	Fluorescein isothiocyanate (FITC) anti-mouse Toll-like receptor 9 (CD289, TLR9, TLR-9)	488	518	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

M9.D6 is generated against a peptide derived from the extracellular portion of mouse TLR9. Predominantly expressed as an intracellular protein, TLR9 is a ~115-120 kDa molecule which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9+ B cells. When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on RBC-lysed mouse splenocytes stained for intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Further studies are needed to determine the relationship between mRNA expression and protein detection.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This M9.D6 antibody has been reported for use in intracellular flow cytometric analysis, and immunoblotting (WB).

Applications Tested

The M9.D6 antibody has been tested by intracellular flow cytometric analysis of fixed and permeabilized mouse TLR9 transfected cells. This can be used at less than or equal to 0.5 ug per million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ahmad-Nejad P, Hacker H, Rutz M, Bauer S, Vabulas RM, Wagner H. 2002. Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol. 32(7): 1958-68.

Fukata M, Breglio K, et al. 2008. The Myeloid Differentiation Factor 88 (MyD88) Is Required for CD4+ T Cell Effector Function in a Murine Model of Inflammatory Bowel Disease. J Immunol. 180(3):1886-94. (M9.D6, IC Flow, PubMed)

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4321  Biotin Rat IgG2a Isotype Control
Cat. 14-8185  Recombinant B18R protein (Vaccinia Virus-Encoded Neutralizing Type I Interferon Receptor; Type I IFN inhibitor)
Cat. 34-8185  Carrier-Free Recombinant B18R protein (Vaccinia Virus-Encoded Neutralizing Type I Interferon Receptor; Type I IFN inhibitor)
Cat. 11-8317  Fluorescein isothiocyanate (FITC) mouse anti-human IFN-alpha2 (clone 225.C)
Cat. 12-8317  Phycoerythrin (PE) mouse anti-human IFN-alpha2 (clone 225.C)
Cat. 17-8317  Allophycocyanin (APC) mouse anti-human IFN-alpha2 (clone 225.C)