human BLyS antibody, Biotin conjugate

Contents: Biotin anti-human BLyS (CD257, BAFF)
Catalog Number: 13-9017
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 1D6
Isotype: Mouse IgG1, κ
HLDA No.: N/A

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13-9017-80

25 ug

13-9017-82

100 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9017	Affinity Purified anti-human BLyS (CD257, BAFF)	N/A	N/A	FC IHC WB
13-9017	Biotin anti-human BLyS (CD257, BAFF)	N/A	N/A	FC
11-9017	FITC anti-human BLyS (CD257, BAFF)	488	518	FC
12-9017	PE anti-human BLyS (CD257, BAFF)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 1D6 monoclonal antibody reacts with human BLyS (B lymphocyte stimulator), also known as BAFF (B cell activating factor from the TNF family), TALL-1 (TNF and apoptosis leukocyte-expressed ligand-1), THANK (TNF homologue that activates apoptosis, nuclear factor KappaB and c-Jun NH2-terminal kinase) and zTNF4. BLyS, a member of the TNF family, is a type II membrane protein that exists in both membrane-bound and soluble forms. Expression of membrane-bound BLyS on monocytes lineage is regulated by IFN-γ. BLyS acts as a potent B cell growth factor and costimulator of Ig production. BLyS transgenic mice develop severe B cell hyperplasia and autoimmune lupus-like disease characterized by the presence of autoantibodies against nuclear antigens and immune complexes deposited in the kidney. Data from animal models imply a role for BLyS in human autoimmune diseases. To date BCMA, TACI and BR3 have been identified as receptors for BLyS. APRIL, another TNF family member, and BLyS can form active heterotrimeric molecules when coexpressed and these circulating heterotrimers are present in serum samples from patients with systemic immune-based rheumatic diseases. Furthermore, it has been reported that BLyS and APRIL, share BCMA and TACI as their receptors.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 1D6 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 1D6 antibody has been tested by flow cytometric analysis of human BLyS transfected cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kayagaki, N., M. Yan, et al. 2002. BAFF/BLyS receptor 3 binds the B cell survival factor BAFF ligand through a discrete surface loop and promotes processing of NF-kappaB2. Immunity 17(4): 515-24.
Roschke, V., S. Sosnovtseva, et al. 2002. BLyS and APRIL form biologically active heterotrimers that are expressed in patients with systemic immune-based rheumatic diseases. J Immunol 169(8): 4314-21.
Hsu, B. L., S. M. Harless, et al. 2002. Cutting edge: BLyS enables survival of transitional and mature B cells through distinct mediators. J Immunol 168(12): 5993-6.
Yan, M., J. R. Brady, et al. 2001. Identification of a novel receptor for B lymphocyte stimulator that is mutated in a mouse strain with severe B cell deficiency. Curr Biol 11(19): 1547-52.
Hase H, Kanno Y, Kojima M, Hasegawa K, Sakurai D, Kojima H, Tsuchiya N, Tokunaga K, Masawa N, Azuma M, Okumura K, Kobata T. 2004. BAFF/BLyS can potentiate B-cell selection with the B-cell coreceptor complex. Blood. 103(6):2257-65.

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