Biotin anti-human TRA-1-81 (podocalyxin)

Contents: Biotin anti-human TRA-1-81 (anti-human Podocalyxin, glycosylation-specific epitope)
Catalog Number: 13-8883
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: TRA-1-81
Isotype: Mouse IgM

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
13-8883-80	25 ug	$80
13-8883-82	100 ug	$140

*International customers: Please contact your distributor for region specific pricing.

Staining of 2102Ep human embryonal carcinoma cells with staining buffer (autofluorescence) (open histogram) or 0.125 μg of Biotin anti-human TRA-1-81 (colored histogram) followed by Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-8883	Phycoerythrin (PE) anti-human TRA-1-81 (podocalyxin)	488	575	FC
14-8883	Affinity Purified anti-human TRA-1-81 (podocalyxin)	N/A	N/A	FC IHC IP WB
13-8883	Biotin anti-human TRA-1-81 (podocalyxin)	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The TRA-1-81 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-81 antibody is lost upon cell differentiation. The TRA-1-81 epitope is resistant to neuraminidase digestion, unlike the epitope recognized by the related TRA-1-60 antibody. The TRA-1-81 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This TRA-1-81 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This TRA-1-81 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schopperle WM, DeWolf WC. The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells. 2007 Mar;25(3):723-30. (TRA-1-81, WB, PubMed)

Xu C, Inokuma MS, Denham J, Golds K, Kundu P, Gold JD, Carpenter MK. Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol. 2001 Oct;19(10):971-4. (PubMed)

Badcock G, Pigott C, Goepel J, Andrews PW. The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res. 1999 Sep 15;59(18):4715-9. (TRA-1-81, WB, PubMed)

Andrews PW, Banting G, Damjanov I, Arnaud D, Avner P. Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma. 1984 Winter;3(4):347-61. (PubMed)

Related Products

Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-8813    Affinity Purified anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1) (clone eBioMC-480 (MC-480))
Cat. 14-8833    Affinity Purified anti-human/mouse SSEA-3 (stage-specific embryonic antigen-3) (clone eBioMC-631 (MC-631))
Cat. 14-8843    Affinity Purified anti-human SSEA-4 (stage-specific embryonic antigen-4) (clone eBioMC-813-70 (MC-813-70))
Cat. 12-8863    Phycoerythrin (PE) anti-human TRA-1-60 (podocalyxin) (clone TRA-1-60)
Cat. 13-8863    Biotin anti-human TRA-1-60 (podocalyxin) (clone TRA-1-60)
Cat. 14-8863    Affinity Purified anti-human TRA-1-60 (podocalyxin) (clone TRA-1-60)