Biotin anti-human SSEA-4 (stage-specific embryonic antigen-4) antibody: biotin conjugate

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Contents: Biotin anti-human SSEA-4 (stage-specific embryonic antigen-4)
Catalog Number: 13-8843
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBioMC-813-70 (MC-813-70)
Isotype: Mouse IgG3

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
13-8843-80	25 ug	$140
13-8843-82	100 ug	$275

*International customers: Please contact your distributor for region specific pricing.

Staining of 2102ep cells with staining buffer (autofluorescence) (open histogram) or 0.5 μg of Biotin anti-human SSEA-4 (MC-813-70) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-8843	Phycoerythrin (PE) anti-human SSEA-4 (stage-specific embryonic antigen-4)	488	575	FC
53-8843	Alexa Fluor® 488 anti-human SSEA-4 (stage-specific embryonic antigen-4)	488	519	FC
14-8843	Affinity Purified anti-human SSEA-4 (stage-specific embryonic antigen-4)	N/A	N/A	FC IHC
13-8843	Biotin anti-human SSEA-4 (stage-specific embryonic antigen-4)	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioMC-813-70 (MC-813-70) antibody has been tested by flow cytometric analysis of the 2102Ep cell line. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Gang EJ, Bosnakovski D, Figueiredo CA, Visser JW, Perlingeiro RC. SSEA-4 identifies mesenchymal stem cells from bone marrow. Blood. 2007 Feb 15;109(4):1743-51.

Kannagi R, Cochran NA, Ishigami F, Hakomori S, Andrews PW, Knowles BB, Solter D. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J. 1983;2(12):2355-61.

Krupnick JG, Damjanov I, Damjanov A, Zhu ZM, Fenderson BA. Globo-series carbohydrate antigens are expressed in different forms on human and murine teratocarcinoma-derived cells. Int J Cancer. 1994 Dec 1;59(5):692-8.

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