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Mouse Cellular Antigens Chart  Human Cellular Antigens Chart

Contents: Biotin anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1) Catalog Number: 13-8813 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 4°C. DO NOT FREEZE. Clone: eBioMC-480 (MC-480) Isotype: Mouse IgM Prices for This Product* Cat. No. Size Price Qty Action 13-8813-80 25 ug $140 13-8813-82 100 ug $275 *International customers: Please contact your distributor for region specific pricing.

Staining of the F9 cell line with staining buffer (autofluorescence) (open histogram) or 0.25 μg of Biotin anti-mo/hu SSEA-1 (MC-480) (colored histogram) followed by Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-8813	Phycoerythrin (PE) anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1)	488	575	FC
13-8813	Biotin anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1)	N/A	N/A	FC
14-8813	Affinity Purified anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1)	N/A	N/A	FC  IHC  IP
51-8813	Alexa Fluor® 647 anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1)	633	668	FC
53-8813	Alexa Fluor® 488 anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1)	488	519	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. SSEA-1 is associated with cell adhesion, migration and differentiation.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.

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Applications Tested

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This eBioMC-480 (MC-480) antibody has been tested by flow cytometric analysis of the F9 cell line. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Anjos-Afonso F, Bonnet D. Nonhematopoietic/endothelial SSEA-1+ cells define the most primitive progenitors in the adult murine bone marrow mesenchymal compartment. Blood. 2007 Feb 1;109(3):1298-306. (PubMed)

Fenderson BA, De Miguel MP, Pyle AD, Donovan PJ. Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens. Methods Mol Biol. 2006;325:207-24. (PubMed)

Solter D, Knowles BB. Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1). Proc Natl Acad Sci U S A. 1978 Nov;75(11):5565-9. (PubMed)

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Related Products

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Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)