human TNFa antibody, Biotin conjugate

Contents: Biotin anti-human TNF-α
Catalog Number: 13-7349
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: MAb11
Isotype: Mouse IgG1, κ

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13-7349-81

50 ug

13-7349-85

500 ug

Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. The cells were surface stained with FITC anti-human CD4 (RPA-T4) and intracellularly stained with PE anti-human TNF-a (Mab11).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7349	Affinity Purified anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	IC Flow
13-7349	Biotin anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	ELISA det ELISPOT det
11-7349	FITC anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	518	IC Flow
12-7349	PE anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	575	IC Flow
17-7349	APC anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	660	IC Flow
25-7349	Phycoerythrin-Cy7 (PE-Cy7) anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	760	IC Flow
51-7349	Alexa Fluor® 647 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	668	IC Flow
53-7349	Alexa Fluor® 488 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	519	IC Flow
57-7349	Pacific Blue® anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	402	421	IC Flow
45-7349	PerCP-Cy5.5 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (PE-Cy5.5 replacement)	488	690	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF-α ), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4⁺ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MAb11 antibody has been reported for use in detection of human TNF-α by ELISA.

Applications Tested

The MAb11 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human TNF-alpha (TNF-a, TNFa) in combination with the purified MAb1 (14-7348) antibody for capture and recombinant human TNFa (14-8329) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

References

Danis, V. A., G. M. Franic, et al. (1991). "Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 and TNF-alpha by human monocytes." Clin Exp Immunol 85(1): 143-50.
Rathjen, D. A., K. Cowan, et al. (1991). "Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors." Mol Immunol 28(1-2): 79-86.

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