Product Information

Contents: Biotin anti-mouse/rat TNF-alpha (TNF-α)
Catalog Number: 13-7341
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7341	Affinity Purified anti-mouse/rat TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	IVCCA
13-7341	Biotin anti-mouse/rat TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) polyclonal	N/A	N/A	ELISA det ELISPOT det

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Description

The affinity purified polyclonal rabbit antibody reacts with mouse and rat tumor necrosis factor-alpha. Mouse TNF-α is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4 T cells, and NK cells. A 26 kDa form is expressed as a membrane-bound molecule. TNF-α is cytolytic and plays an important role in immune regulation. Bioactive TNF-α exists as a homotrimer.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in ELISA and ELISPOT.

Applications Tested

The biotinylated rabbit anti-mouse/rat TNF-α polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse or rat TNF-α in combination with the purified anti-mouse/rat TNF-α monoclonal antibody, clone TN3.19 (14-7423) for capture and recombinant mouse TNF-α (14-8321) or rat TNF-α (14-8321) as the standard. A suitable range of concentrations of this biotinylated antibody for ELISA detection is 0.5 – 2.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml (mouse TNF-α) or 5000 – 30 pg/ml (rat TNF-α) should be included in each ELISA plate. Recognition of mouse TNF-α by this assay is not interfered with by 1000x excess soluble TNF-α receptor type I or II.

References

Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

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