Print Page

Adobe PDF

Contents: Biotin anti-mouse/rat TNF-alpha (TNF-α) Catalog Number: 13-7341 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. DO NOT FREEZE. Clone: Polyclonal Isotype: Rabbit IgG Prices for This Product* Cat. No. Size Price Qty Action 13-7341-81 50 ug $100 13-7341-85 500 ug $295 *International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-7341	Biotin anti-mouse/rat TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) polyclonal	N/A	N/A	ELISA det  ELISPOT det
14-7341	Affinity Purified anti-mouse/rat TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	IVCCA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

---

Description

---

The affinity purified polyclonal rabbit antibody reacts with mouse and rat tumor necrosis factor-alpha. Mouse TNF-α is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4 T cells, and NK cells. A 26 kDa form is expressed as a membrane-bound molecule. TNF-α is cytolytic and plays an important role in immune regulation. Bioactive TNF-α exists as a homotrimer.

---

Applications Reported

---

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in ELISA and ELISPOT.

---

Applications Tested

---

The biotinylated rabbit anti-mouse/rat TNF-α polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse or rat TNF-α in combination with the purified anti-mouse/rat TNF-α monoclonal antibody, clone TN3.19 (14-7423) for capture and recombinant mouse TNF-α (14-8321) or rat TNF-α (14-8321) as the standard. A suitable range of concentrations of this biotinylated antibody for ELISA detection is 0.5 – 2.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml (mouse TNF-α) or 5000 – 30 pg/ml (rat TNF-α) should be included in each ELISA plate. Recognition of mouse TNF-α by this assay is not interfered with by 1000x excess soluble TNF-α receptor type I or II.

---

References

---

Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

---

Related Products

---

Cat. 00-0000    eBioscience 10X ELISA Coating Buffer
Cat. 44-2404    Nunc MaxiSorp™ flat-bottom 96 well plate
Cat. 18-4100    Avidin Horse Radish Peroxidase (AV-HRP)
Cat. 00-4202    eBioscience ELISA Diluent Solution, 5X
Cat. 00-4203    Super AquaBlue ELISA Substrate
Cat. 11-7321    FITC anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone MP6-XT22)
Cat. 12-7321    PE anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone MP6-XT22)
Cat. 14-7321    Affinity Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone MP6-XT22)
Cat. 17-7321    APC anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone MP6-XT22)
Cat. 19-7321    Cy5 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone MP6-XT22)
Cat. 16-7322    Functional Grade Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone MP6-XT3)
Cat. 88-7324    Mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) ELISA Ready-SET-Go!
Cat. 14-7325    Affinity Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone 1F3F3D4)
Cat. 11-7423    Fluorescein (FITC) anti-mouse/rat TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone TN3-19)
Cat. 12-7423    Phycoerythrin (PE) anti-mouse/rat TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone TN3-19)
Cat. 14-7423    Affinity Purified anti-mouse/rat TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone TN3-19)
Cat. 16-7423    Functional Grade Purified anti-mouse/rat TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone TN3-19)
Cat. 14-8321    Recombinant Mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)