Biotin anti-mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) antibody: biotin conjugate

Contents: Biotin anti-mouse GM-CSF
Catalog Number: 13-7332
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: MP1-31G6
Isotype: Rat IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
13-7332-81	50 ug	$95
13-7332-85	500 ug	$260

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-7332	Biotin anti-mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF)	N/A	N/A	ELISA det ELISPOT det

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MP1-31G6 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF), a 14 kDa cytokine secreted by activated T cells and macrophages.

Applications Reported

For research use only, not for diagnostic or therapeutic use. MP1-31G6 has been reported for use in detection of mouse GM-CSF by ELISA.

Applications Tested

The MP1-31G6 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) in combination with the purified MP1-22E9 (14-7331) antibody for capture and recombinant mouse GM-CSF (14-8331) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

References

Sander, B., I. Hoiden, et al. (1993). "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." J Immunol Methods 166(2): 201-14.
Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.

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