TNFa antibody, Biotin conjugate

Contents: Biotin anti-mouse TNF-α cocktail
Catalog Number: 13-7326
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: XT3/XT22
Isotype: Rat IgG1, κ

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13-7326-81

50 ug

13-7326-85

500 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-7326	Biotin anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) Cocktail	N/A	N/A	ELISA det

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Description

The MP6-XT3 and MP6-XT22 antibodies react with mouse tumor necrosis factor-alpha (TNF-α ).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This cocktail antibody has been reported for use in detection of mouse TNF-α by ELISA. Fluorochrome-conjugated formats of monoclonal antibody specific for mouse TNF-α, MP6-XT22, (FITC 11-7321, PE 12-7321, APC 17-7321) are effective for staining of intracellular TNF-α. The Functional Grade purified format of monoclonal antibody specific for mouse TNF-α, MP6-XT3, (cat.16-7322) is effective for neutralizing biological activity of TNF-α.

Applications Tested

The biotinylated MP6-XT3 and MP6-XT22 antibody cocktail has been tested as the detection antibody in a sandwich ELISA for analysis of mouse TNF-α in combination with the purified 1F3F3D4 antibody (14-7325) for capture and recombinant mouse TNFa (14-8321) as the standard. A suitable range of concentrations of this biotinylated antibody for ELISA detection is 0.5 – 2.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.
Recognition of mouse TNF-α by this assay is not interfered with by 1000x excess soluble TNF-α receptor type I or II.

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