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Contents: Biotin anti-mouse TNF-α cocktail Catalog Number: 13-7326 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. DO NOT FREEZE. Clone: XT3/XT22 Isotype: Rat IgG1, κ Prices for This Product* Cat. No. Size Price Qty Action 13-7326-81 50 ug $100 13-7326-85 500 ug $295 *International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-7326	Biotin anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) Cocktail	N/A	N/A	ELISA det

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The MP6-XT3 and MP6-XT22 antibodies react with mouse tumor necrosis factor-alpha (TNF-α ).

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. This cocktail antibody has been reported for use in detection of mouse TNF-α by ELISA. Fluorochrome-conjugated formats of monoclonal antibody specific for mouse TNF-α, MP6-XT22, (FITC 11-7321, PE 12-7321, APC 17-7321) are effective for staining of intracellular TNF-α. The Functional Grade purified format of monoclonal antibody specific for mouse TNF-α, MP6-XT3, (cat.16-7322) is effective for neutralizing biological activity of TNF-α.

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Applications Tested

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The biotinylated MP6-XT3 and MP6-XT22 antibody cocktail has been tested as the detection antibody in a sandwich ELISA for analysis of mouse TNF-α in combination with the purified 1F3F3D4 antibody (14-7325) for capture and recombinant mouse TNFa (14-8321) as the standard. A suitable range of concentrations of this biotinylated antibody for ELISA detection is 0.5 – 2.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.
Recognition of mouse TNF-α by this assay is not interfered with by 1000x excess soluble TNF-α receptor type I or II.

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