Biotin anti-human IFNg (Interferon-gamma, IFN-g) antibody: biotin conjugate

Contents: Biotin anti-human Interferon-γ (IFN-γ)
Catalog Number: 13-7319
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 4S.B3
Isotype: Mouse IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
13-7319-81	50 ug	$95
13-7319-85	500 ug	$260

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7319	FITC anti-human IFNg (Interferon-gamma, IFN-g)	488	518	IC Flow
12-7319	PE anti-human IFNg (Interferon-gamma, IFN-g)	488	575	IC Flow
13-7319	Biotin anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA det ELISPOT
14-7319	Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	IC Flow
17-7319	APC anti-human IFNg (Interferon-gamma, IFN-g)	633	660	IC Flow
25-7319	Phycoerythrin-Cy7 (PE-Cy7) anti-human IFNg (Interferon-gamma, IFN-g)	488	760	IC Flow
45-7319	PerCP-Cy5.5 anti-human IFNg (Interferon-gamma, IFN-g)	488	690	IC Flow
51-7319	Alexa Fluor® 647 anti-human IFNg (Interferon-gamma, IFN-g)	633	668	IC Flow
53-7319	Alexa Fluor® 488 anti-human IFNg (Interferon-gamma, IFN-g)	488	519	IC Flow
57-7319	Pacific Blue® anti-human IFNg (Interferon-gamma, IFN-g)	402	421	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 4S.B3 monoclonal antibody reacts with interferon-γ (IFN-γ). The 4S.B3 antibody is a neutralizing antibody. Human IFN-γ is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-γ in synergy with other cytokines, such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 4S.B3 antibody has been reported for use in ELISPOT, and ELISA.

Applications Tested

The 4S.B3 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human interferon-γ (IFN-γ) in combination with the purified NIB42 (14-7318) or MD-1 (14-7317) antibodies for capture and recombinant human IFN-γ (14-8319) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

References

Meager, A., S. Parti, et al. (1984). "Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon." J Interferon Res 4(4): 619-25.

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