Contents: Biotin anti-mouse IFN-γ
Catalog Number: 13-7311
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE
Clone: XMG1.2
Isotype: Rat IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
 
Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. (Left) The cells were surface-stained with PE anti-mouse CD4 (GK1.5) and intracellularly stained with FITC anti-mouse IFN-g (XMG1.2). (Right) The cells were surface stained with APC anti-mouse CD4 and intracellularly stained with PE anti-mouse IFN-g.
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-7311 |
FITC anti-mouse IFNg (Interferon-gamma, IFN-g) |
488 |
518 |
IC Flow
|
| 12-7311 |
PE anti-mouse IFNg (Interferon-gamma, IFN-g) |
488 |
575 |
IC Flow
|
| 13-7311 |
Biotin anti-mouse IFNg (Interferon-gamma, IFN-g) |
N/A |
N/A |
ELISA
|
| 14-7311 |
Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g) |
N/A |
N/A |
ELISA cap
|
| 16-7311 |
Functional Grade* Purified anti-mouse IFNg (Interferon-gamma, IFN-g) |
N/A |
N/A |
NU
|
| 17-7311 |
APC anti-mouse IFNg (Interferon-gamma, IFN-g) |
633 |
660 |
IC Flow
|
| 19-7311 |
Cy5 anti-mouse IFNg (Interferon-gamma, IFN-g) |
633 |
670 |
IC Flow
|
| 25-7311 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IFNg (Interferon-gamma, IFN-g) |
488 |
760 |
IC Flow
|
| 45-7311 |
PerCP-Cy5.5 anti-mouse IFNg (Interferon-gamma, IFN-g) |
488 |
690 |
IC Flow
|
| 51-7311 |
Alexa Fluor® 647 anti-mouse IFNg (Interferon-gamma, IFN-g) |
633 |
668 |
IC Flow
|
| 53-7311 |
Alexa Fluor® 488 anti-mouse IFNg (Interferon-gamma, IFN-g) |
488 |
519 |
IC Flow
|
| 57-7311 |
Pacific Blue® anti-mouse IFNg (Interferon-gamma, IFN-g) |
402 |
421 |
IC Flow
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The XMG1.2 antibody reacts with mouse interferon-γ. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN-γ is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.
Applications Reported
For research use only, not for diagnostic or therapeutic use. XMG1.2 has been reported for use in capture or detection of mouse IFN-γ by ELISA and immunoblotting (WB).
Applications Tested
The XMG1.2 antibody has been tested by sandwich ELISA for detection of mouse IFNγ in combination with R4-6A2 and can be used at 1 µg/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples."
Immunol Rev 127: 5-24.
Related Products
Cat.
00-0000 eBioscience 10X ELISA Coating Buffer
Cat.
44-2404 Nunc MaxiSorp™ flat-bottom 96 well plate
Cat.
18-4100 Avidin Horse Radish Peroxidase (AV-HRP)
Cat.
00-4202 eBioscience ELISA Diluent Solution, 5X
Cat.
00-4203 Super AquaBlue ELISA Substrate
Cat.
88-7234 Mouse IL-23 (p19/p40, IL23) ELISA Ready-SET-Go!
Cat.
14-7312 Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g) (clone R4-6A2)
Cat.
14-8311 Recombinant Mouse IFNg (Interferon-gamma, IFN-g)
